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In Silico Design of gRNA for CRISPR/Cas9-Mediated Gene Knockout
CRISPR/Cas9 stands as a revolutionary and versatile gene editing technology. At its core, the Cas9 DNA endonuclease is guided with precision by a...
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Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System
Engineering of the mouse germline is a key technology in biomedical research for studying the function of genes in health and disease. Since the...
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Challenges in Eventing Horizontal Gene Transfer
Horizontal gene transfer (HGT) takes place when two organisms share their genetic material. Contrary to the ancestral transfer of genes, viz....
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Interconnected Codons: Unravelling the Epigenetic Significance of Flanking Sequences in CpG Dyads
Hypothesizing that CpG codon dyads, formed by consecutive codons containing a cytosine-guanine pair (NNC-GNN), may play a crucial role in gene...
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7 Mammalian ACAT and DGAT2 gene families
Mammalian isozymes of ACAT, DGAT, and MGAT are encoded by the ACAT and DGAT2 gene families. These enzymes catalyze the synthesis of neutral lipid...
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The Distribution of the Germ Plasm Gene germes among Anurans
AbstractThe germes gene is a marker of germ plasm and primordial germ cells (PGC). It is described for the African clawed frog Xenopus laevis ....
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Gene Editing
Gene editing is a technique of genetic engineering when a particular fragment of DNA is either inserted, replaced, or removed from a genome using...
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Characterization of Gene Regulatory Networks in Plants Using New Methods and Data Types
A major question in plant biology is to understand how plant growth, development, and environmental responses are controlled and coordinated by the...
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Analysis of the complete genome sequences of Clostridium perfringens strains harbouring the binary enterotoxin BEC gene and comparative genomics of pCP13-like family plasmids
BackgroundBEC-producing Clostridium perfringens is a causative agent of foodborne gastroenteritis. It was first reported in 2014, and since then,...
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G4Bank: A database of experimentally identified DNA G-quadruplex sequences
G-quadruplex (G4), a non-canonical nucleic acid structure, has been suggested to play a key role in important cellular processes including...
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Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases
Programmable genome integration of large, diverse DNA cargo without DNA repair of exposed DNA double-strand breaks remains an unsolved challenge in...
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Genome Editing of Gene Families for Crop Improvement
Crop improvement has been a long-standing focus of agricultural research, aiming to enhance nutritional richness, aroma, visual appeal, and yield to...
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Differential Gene Expression During Somatic Embryogenesis
Somatic embryogenesis is a complex developmental program in which somatic cells are induced for a commitment towards forming totipotent...
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SCORPiOs, a Novel Method to Reconstruct Gene Phylogenies in the Context of a Known WGD Event
Phylogenetic gene trees recapitulate the evolutionary history of genes across species, forming an essential framework for comparative genomic...
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Complete chloroplast genome sequences of the ornamental plant Prunus cistena and comparative and phylogenetic analyses with its closely related species
BackgroundPrunus cistena is an excellent color leaf configuration tree for urban landsca** in the world, which has purplish red leaves, light pink...
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IS6 family insertion sequences promote optrA dissemination between plasmids varying in transfer abilities
AbstractPlasmids are the primary vectors for intercellular transfer of the oxazolidinone and phenicol cross-resistance gene optrA , while insertion...
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AGO, a Framework for the Reconstruction of Ancestral Syntenies and Gene Orders
Reconstructing ancestral gene orders from the genome data of extant species is an important problem in comparative and evolutionary genomics. In a...
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Gene Targeting in Rabbits: Single-Step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids
The development of genome editing technology has allowed gene disruptions to be achieved in various animal species and has been beneficial to many...
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Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences
The opportunistic fungal pathogen Candida albicans damages host cells via its peptide toxin, candidalysin. Before secretion, candidalysin is embedded...
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DeepConsensus improves the accuracy of sequences with a gap-aware sequence transformer
Circular consensus sequencing with Pacific Biosciences (PacBio) technology generates long (10–25 kilobases), accurate ‘HiFi’ reads by combining...
