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Using Pull Down Strategies to Analyze the Interactome of Peroxisomal Membrane Proteins in Human Cells
Different pull-down strategies were successfully applied to gain novel insight into the interactome of human membrane-associated proteins. Here, we...
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The NADP+-dependent glutamate dehydrogenase Gdh1 is subjected to glucose starvation-induced reversible aggregation that affects stress resistance in yeast
The yeast Saccharomyces cerevisiae has two isoforms of NADP + -dependent glutamate dehydrogenase (Gdh1 and Gdh3) that catalyze the synthesis of...
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Members of the nitronate monooxygenase gene family from Metarhizium brunneum are induced during the process of infection to Plutella xylostella
Metarhizium species are the most abundant fungi that can be isolated from soil, with a well-known biopesticide capacity. Metarhizium recognizes their...
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The Woronin Body: A Fungal Organelle Regulating Multicellularity
In filamentous fungal species belonging to the Phyla Ascomycota and Basidiomycota, hyphae are compartmentalized into distinct cells by the formation...
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Neutralization of mobile antiviral small RNA through peroxisomal import
In animals, certain viral proteins are targeted to peroxisomes to dampen the antiviral immune response mediated by these organelles
1 –3 . In plants,...
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Multi-localized Proteins: The Peroxisome-Mitochondria Connection
Peroxisomes and mitochondria are dynamic, multifunctional organelles that play pivotal cooperative roles in the metabolism of cellular lipids and...
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Cell Death or Survival Against Oxidative Stress
Peroxisomes contain anabolic and catabolic enzymes including oxidases that produce hydrogen peroxide as a by-product. Peroxisomes also contain...
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Alternative splicing affects the targeting sequence of peroxisome proteins in Arabidopsis
Key messageA systematic analysis of the Arabidopsis genome in combination with localization experiments indicates that alternative splicing affects...
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Multiple Localization by Functional Translational Readthrough
In a compartmentalized cell, correct protein localization is crucial for function of virtually all cellular processes. From the cytoplasm as...
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Proteome of Plant Peroxisomes
Plant peroxisomes are required for a number of fundamental physiological processes, such as primary and secondary metabolism, development and stress...
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Neue Isoformen für alte Enzyme durch translationalen readthrough
Malate and lactate dehydrogenase (MDH and LDH) are linked to a new and unusual class of proteins in mammals: proteins with an extension generated by...
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Fungal Peroxisomes Proteomics
Peroxisomes in fungi are involved in a huge number of different metabolic processes. In addition, non-metabolic functions have also been identified....
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Peroxisome Mitochondria Inter-relations in Plants
A large amount of ultrastructural, biochemical and molecular analysis indicates that peroxisomes and mitochondria not only share the same subcellular...
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A Role for RNS in the Communication of Plant Peroxisomes with Other Cell Organelles?
Plant peroxisomes are organelles with a very active participation in the cellular regulation of the metabolism of reactive oxygen species (ROS)....
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Peroxisomes and Cellular Oxidant/Antioxidant Balance: Protein Redox Modifications and Impact on Inter-organelle Communication
Disturbances in cellular redox balance have been associated with pro-aging mechanisms and increased risk for various chronic disease states. Multiple...
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A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery
Here we describe a protocol to dissect the peroxisomal matrix protein import pathway using a cell-free in vitro system. The system relies on a...
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Transcriptome, proteome and draft genome of Euglena gracilis
BackgroundPhotosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility,...
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The curious case of peroxiredoxin-5: what its absence in aves can tell us and how it can be used
BackgroundPeroxiredoxins are ubiquitous thiol-dependent peroxidases that represent a major antioxidant defense in both prokaryotic cells and...
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Temperature Stress and Redox Homeostasis: The Synergistic Network of Redox and Chaperone System in Response to Stress in Plants
A remarkable number of strategies has been developed by living organisms to mitigate conflict with environmental changes. The global environment...