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Sequencing: 10X Genomics 3′ HT Assay for Gene Expression
Single-cell RNA sequencing supports the isolation of individual cells and barcoding of cDNA, specific to each cell of origin. Subsequent sequencing...
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Transcriptomic analysis of paternal behaviors in prairie voles
BackgroundThe importance of fathers’ engagement in care and its critical role in the offspring’s cognitive and emotional development is now well...
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Cancer biology deciphered by single-cell transcriptomic sequencing
Tumors are complex ecosystems in which heterogeneous cancer cells interact with their microenvironment composed of diverse immune, endothelial, and...
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Integrated transcriptomic and metabolomic analyses reveals anthocyanin biosynthesis in leaf coloration of quinoa (Chenopodium quinoa Willd.)
BackgroundQuinoa leaves demonstrate a diverse array of colors, offering a potential enhancement to landscape aesthetics and the development of...
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Sample and Library Preparation for PacBio Long-Read Sequencing in Grapevine
PacBio long-read sequencing is a third-generation technology that generates long reads up to 20 kilobases (kb), unlike short-read sequencing...
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Transcriptomic basis of sex loss in the pea aphid
BackgroundTransitions from sexual to asexual reproduction are common in eukaryotes, but the underlying mechanisms remain poorly known. The pea aphid—
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Transcriptomic changes in single yeast cells under various stress conditions
BackgroundThe stress response of Saccharomyces cerevisiae has been extensively studied in the past decade. However, with the advent of recent...
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Transcriptomic response study of brittle star Ophiothrix exigua to sediment burial
During the process of seabed mining, sediment redeposition will lead to the burial of organisms in the seabed environment, resulting in the death of...
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Single-cell transcriptomic atlas of goat ovarian aging
BackgroundThe ovaries are one of the first organs that undergo degenerative changes earlier in the aging process, and ovarian aging is shown by a...
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Next Generation Sequencing in Metagenomics and Metatranscriptomics
This study explores metagenomics and metatranscriptomics, delving into the intricacies of understanding complex microbial communities through...
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Standardization of Single-Cell RNA-Sequencing Analysis Workflow to Study Drosophila Ovary
Developments in single-cell technology have considerably changed the way we study biology. Significant efforts have been made over the last few years...
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Comparative transcriptomic analysis of the different developmental stages of ovary in the cuttlefish Sepia pharaonis
The cuttlefish, Sepia pharaonis , is characterized by rapid growth and strong disease resistance, making it an important commercially farmed...
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COVID-19 and influenza infections mediate distinct pulmonary cellular and transcriptomic changes
SARS-CoV-2 infection can cause persistent respiratory sequelae. However, the underlying mechanisms remain unclear. Here we report that sub-lethally...
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Spatial transcriptomic brain imaging reveals the effects of immunomodulation therapy on specific regional brain cells in a mouse dementia model
Increasing evidence of brain-immune crosstalk raises expectations for the efficacy of novel immunotherapies in Alzheimer’s disease (AD), but the lack...
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Transcriptomic Analysis in the Sea Anemone Nematostella vectensis
The sea anemone Nematostella vectensis is an emerging research model to study embryonic development and regeneration at the molecular and global...
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Combined transcriptomic and pangenomic analyses guide metabolic amelioration to enhance tiancimycins production
AbstractExploration of high-yield mechanism is important for further titer improvement of valuable antibiotics, but how to achieve this goal is...
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RNA Sequencing (RNA-seq)
RNA sequencing (or popularly known as RNA-seq) or whole transcriptome shortgun sequencing (WTSS) is a useful next-generation sequencing (NGS)...
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Transcriptomic analysis of develo** sorghum grains to detect genes related to cell wall biosynthesis and remodelling
ObjectiveSorghum ( Sorghum bicolor (L.) Moench) is the fifth most important grain produced in the world. Interest for cultivating sorghum is...
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Hormonal regulation changes in shrimp infected with Enterocytozoon hepatopenaei: a transcriptomic analysis
Enterocytozoon hepatopenaei (EHP) is a fungal pathogen that causes hepatopancreatic microsporidiosis (HPM) in shrimp, resulting in significant...
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Unveiling the transcriptomic complexity of Miscanthus sinensis using a combination of PacBio long read- and Illumina short read sequencing platforms
BackgroundMiscanthus sinensis Andersson is a perennial grass that exhibits remarkable lignocellulose characteristics suitable for sustainable...
