Search
Search Results
-
Transcription Factors and Their Regulatory Role in Plant Defence Response
Plant diseases critically affect the growth and productivity with an eventual impact on global economy, food safety, and security. The regulation of...
-
Preparation of tRNAArg for Arginylation Assay by In Vitro Transcription
This chapter describes the preparation of tRNAArg by in vitro transcription. tRNA produced by this method can be efficiently utilized for in vitro...
-
A Directed Evolution Protocol for Engineering Minimal Transcription Factors, Based on CIS Display
Directed evolution is an efficient strategy for obtaining desired biomolecules. Since the 1990s, the emergence of display techniques has enabled...
-
A Controlled Transcription-Driven Light-Up Aptamer Amplification for Nucleoside Triphosphate Detection
In this study, we introduced a simple method for detecting nucleoside triphosphates (NTPs) using controlled transcription-driven light-up aptamer...
-
The Effect of the TacA Knockout Transcription Factor on the cbhI Gene Transcription and Expression in the Filamentous Fungus Strain Penicillium verruculosum
AbstractThe tacA gene encoding the TacA repressor protein is cloned by the method for “walking” in uncloned DNA from the genomic DNA of the fungus Pen...
-
Thermostable in vitro transcription-translation compatible with microfluidic droplets
BackgroundIn vitro expression involves the utilization of the cellular transcription and translation machinery in an acellular context to produce one...
-
Transcription regulation strategies in methylotrophs: progress and challenges
As a promising industrial microorganism, methylotroph is capable of using methane or methanol as the sole carbon source natively, which has been...
-
Histone tyrosine sulfation by SULT1B1 regulates H4R3me2a and gene transcription
Tyrosine sulfation is a common posttranslational modification in mammals. To date, it has been thought to be limited to secreted and transmembrane...
-
Human milk exosome-derived circDNAJB6 improves bronchopulmonary dysplasia model by promoting DNAJB6 gene transcription
To verify the protective effect of circDNAJB6 on Bronchopulmonary dysplasia (BPD) cell and animal models and to explore the possible mechanism of its...
-
Cyclic AMP-regulatory element-binding protein: a novel UV-targeted transcription factor in skin cancer
AbstractCommon therapeutics in relation to melanoma and non-melanoma cancers include the use of kinase inhibitors. The long-term benefits of kinases,...
-
CEBPA Restrains the Malignant Progression of Breast Cancer by Prompting the Transcription of SOCS2
The suppressor of cytokine signaling 2 (SOCS2) has been identified to act as a tumor suppressor in breast cancer (BC) progression. However, the...
-
FOXO3 Inhibits the Cisplatin Resistance and Progression of Melanoma Cells by Promoting CDKN1C Transcription
BackgroundForkhead box O3 (FOXO3) and cyclin dependent kinase inhibitor 1 C Gene (CDKN1C) have been shown to be involved in the melanoma process, but...
-
Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection
Aichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with...
-
Investigation of the enhancement for Echinocandin B fermentation with methyl oleate from transcription level
Echinocandin B (ECB) is the key precursor compound of the antifungal drug Anidulafungin. The effects of the five precursor amino acids on ECB...
-
Spatiotemporal and global profiling of DNA–protein interactions enables discovery of low-affinity transcription factors
Precise dissection of DNA–protein interactions is essential for elucidating the recognition basis, dynamics and gene regulation mechanism. However,...
-
High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription
In vitro transcription (IVT) is an essential technique for RNA synthesis. Methods for the accurate and rapid screening of IVT conditions will...
-
Tuning transcription factor DegU for develo** extracellular protease overproducer in Bacillus pumilus
BackgroundGlobal transcription machinery engineering (gTME) is an effective approach employed in strain engineering to rewire gene expression and...
-
YY1 Contributes to the Inflammatory Responses of Mycobacterium tuberculosis‐Infected Macrophages Through Transcription Activation-Mediated Upregulation TLR4
Tuberculosis (TB) is a chronic respiratory infectious disease and is induced by Mycobacterium tuberculosis (M.tb) infection. Macrophages serve as the...
-
Functional Mutations in the microRNA-155 Promoter Modulate its Transcription Efficiency and Expression
Limited research has been conducted on porcine miR-155 promoters, and previous study from our group have identified two haplotypes (TT and CC) in...
-
A CRISPR-Cas12a-based electrochemical biosensor for the detection of microphthalmia-associated transcription factor
A novel electrochemical biosensor that combines the CRISPR-Cas12a system with a gold electrode is reported for the rapid and sensitive detection of...
