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Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
BackgroundThe methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However,...
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Rapid Genome Modification in Serratia marcescens Through Red Homologous Recombination
Despite the great potential of Serratia marcescens in industrial applications, lack of powerful genetic modification tools limits understanding of...
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Characterizing the portability of phage-encoded homologous recombination proteins
Efficient genome editing methods are essential for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method...
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An algorithm to classify homologous series within compound datasets
Homologous series are groups of related compounds that share the same core structure attached to a motif that repeats to different degrees. Compounds...
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CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion
BackgroundHalophiles possess several unique properties and have broad biotechnological applications including industrial biotechnology production. Halomonas...
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Iterative gene integration mediated by 26S rDNA and non-homologous end joining for the efficient production of lycopene in Yarrowia lipolytica
Because of its potent antioxidant effects, lycopene has been used in various industries including, but not limited to, food, medical, and cosmetic...
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Targeted Genome Editing of Virulent Pseudomonas Phages Using CRISPR-Cas3
The vast number of unknown phage-encoded ORFan genes and limited insights into the genome organization of phages illustrate the need for efficient...
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Influence of ku70 Gene Knockout on the Transformation Frequency of Penicillium verruculosum Mycelial Fungi
AbstractTo increase the frequency of homologous recombination (HR) during the transformation of the industrial Penicillium verruculosum 221-151...
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Construction of Novel Live Genetically Modified BCG Vaccine Candidates Using Recombineering Tools
One of the strategies for the construction of live vaccine candidates is through the generation of genetically defined isogenic strains, containing...
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Bxb1-att Site-Specific Recombination System-Mediated Autoexcision to Prevent Environmental Transgene Escape
Transgenic plants are obtained experimentally with low frequency. Scientists use selectable marker genes (SMGs) and selection agents to select...
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Evolution of Transgenic Technology: From Random Transgenesis to Precise Genome Editing
New techniques in molecular biology help us for better understanding of biological processes. As biologists we resort to powerful approaches for...
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Optimizing multicopy chromosomal integration for stable high-performing strains
The copy number of genes in chromosomes can be modified by chromosomal integration to construct efficient microbial cell factories but the resulting...
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Rational construction of a high-quality and high-efficiency biosynthetic system and fermentation optimization for A82846B based on combinatorial strategies in Amycolatopsis orientalis
BackgroundOritavancin is a new generation of semi-synthetic glycopeptide antibiotics against Gram-positive bacteria, which served as the first and...
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Cloning Strategies for the Generation of Recombinant Capripoxvirus Through the Use of Screening and Selection Markers
The ability to manipulate capripoxvirus through gene knockouts and gene insertions has become an increasingly valuable research tool in elucidating...
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Bacillus integrative plasmid system combining a synthetic gene circuit for efficient genetic modifications of undomesticated Bacillus strains
BackgroundOwing to CRISPR-Cas9 and derivative technologies, genetic studies on microorganisms have dramatically increased. However, the CRISPR-Cas9...
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A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Gene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological...
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Gene Expression
During the normal functioning of living organisms, proteins are expressed according to well-regulated mechanisms to meet specific needs, this process...
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Enhanced production of d-pantothenic acid in Corynebacterium glutamicum using an efficient CRISPR–Cpf1 genome editing method
BackgroundCorynebacterium glutamicum has industrial track records for producing a variety of valuable products such as amino acids. Although...
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Improving Fructooligosaccharide Production via sacC Gene Deletion in Zymomonas mobilis: A Novel Approach for Enhanced Prebiotic Production
Fructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. Industrially, these compounds are...
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In vitro and in vivo recombination of heterologous modules for improving biosynthesis of astaxanthin in yeast
BackgroundAstaxanthin is a kind of tetraterpene and has strong antioxygenic property. The biosynthesis of astaxanthin in engineered microbial chassis...
