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Optimization of three-phase partitioning system for the purification of protease from Calotropis gigantea latex using response surface methodology
The response surface methodology (RSM) was utilized to optimize three-phase partitioning (TPP) parameters for the purification of Calotropis gigantea ...
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Lactoferrin-Chitosan-TPP Nanoparticles: Antibacterial Action and Extension of Strawberry Shelf-Life
The aim of the present study was to produce lactoferrin (L) and chitosan (C) nanoparticles by ionic cross-linking with TPP and thereby increase the...
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Protein Extraction and Purification by Differential Solubilization
The preparation of purified soluble proteins for biochemical studies is essential and the solubility of a protein of interest in various media is...
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Bi2S3/Ti3C2-TPP nano-heterostructures induced by near-infrared for photodynamic therapy combined with photothermal therapy on hypoxic tumors
BackgroundPhotodynamic therapy (PDT) efficacy of bismuth sulfide (Bi 2 S 3 ) semiconductor has been severely restricted by its electron–hole pairs (e − −h + ...
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Hydrogenation of nitroarenes in continuous flow with TPP/Raney Ni
In this work, the method of combining the various ligands with Raney Ni in continuous flow is proposed, in which triphenylphosphine (TPP) can...
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Continuous flow synthesis of meso-substituted porphyrins with inline UV–Vis analysis
Porphyrin derivatives have found diverse applications due to their attractive photophysical and catalytic properties, but remain challenging to...
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Production and Purification of Adeno-Associated Viral Vectors (AAVs) Using Orbitally Shaken HEK293 Cells
Here, we describe methods for the production of adeno-associated viral (AAV) vectors by transient transfection of HEK293 cells grown in serum-free...
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The Utilization of Highly Mineralized Liquid Waste from a Chemical Desalination Water Treatment Plant of a TPP with the Generation of Electrical Energy by Reverse Electrodialysis
AbstractReverse electrodialysis (RED) technology is one of the promising directions of alternative energy based on electrochemical transformations....
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Harnessing ceramic hydroxyapatite as an effective polishing strategy to remove product- and process-related impurities in bispecific antibody purification
Bispecific antibody (bsAb), a novel therapeutic modality, provides excellent treatment efficacy, yet poses numerous challenges to downstream process...
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Thermal Proteome Profiling for Drug Target Identification and Probing of Protein States
Proteins are central drivers of physiological and pathological processes in the cell. Methods evaluating protein functional states are therefore...
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Discovery of metal-binding proteins by thermal proteome profiling
Metal-binding proteins (MBPs) have various and important biological roles in all living species and many human diseases are intricately linked to...
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Multifunctional TPP-PEG-biotin self-assembled nanoparticle drug delivery-based combination therapeutic approach for co-targeting of GRP78 and lysosome
BackgroundIn this study, a multifunctional tetraphenylporphyrin (TPP) conjugated polyethylene glycol with biotin (TPP-PEG-biotin) as a photo-dynamic...
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Evaluation of Three-Phase Partitioning for Efficient and Simultaneous Isolation of Immunomodulatory Polysaccharides and Proteins from Lentinula Edodes Mushroom
Lentinula edodes is one of the most popular edible mushrooms and is also widely used as a health food. Polysaccharides (PS) and proteins are two...
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Rational construction of tetraphenylporphyrin/bismuth oxybromide nanocomposite with accelerated interfacial charge transfer for promoted visible-light-driven degradation of antibiotics
Photocatalysis plays a significant role in the field of environment purification and energy conversion. In this work, a series of...
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Fabricating of flame retardant and antibacterial lyocell fabric based on thiamine pyrophosphate modification
To improve the flame retardant and antibacterial properties of lyocell samples, thiamine pyrophosphate (TPP), a kind of water-soluble vitamin...
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All-in-one functional supramolecular nanoparticles based on pillar[5]arene for controlled generation, storage and release of singlet oxygen
The storage and controlled release of singlet oxygen ( 1 O 2 ) have attracted increasing attention due to the wide application and microsecond lifetime...
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Boosting thermal stability and crystallization of closed-loop-recyclable biodegradable poly(p-dioxanone) by end-group regulation
A chemically closed-loop-recyclable biodegradable polymer, poly( p -dioxanone) (PPDO), is one of the ideal candidates for single-use plastic products...
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Entrapment of Papain in Chitosan–Polyethylene Glycol Hybrid Nanohydrogels: Presenting a Model for Protein Delivery Systems
In this study, the process of manufacturing nanohydrogels containing papain and how to release it was investigated. Chitosan nanohydrogels and...
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Combining Ni3P and Lewis Acid–Base Pair as a High-Performance Catalyst for Amination of 1-Octanol
Ni phosphide and Lewis acid–base pair site of Al 2 O 3 were combined as highly efficient catalyst for direct amination of 1-octanol to 1-octylamine. Due...
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Mitochondria-targeting multifunctional nanoplatform for cascade phototherapy and hypoxia-activated chemotherapy
Despite considerable progress has been achieved in hypoxia-associated anti-tumor therapy, the efficacy of utilizing hypoxia-activated prodrugs alone...
