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Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons
Cultured primary neurons are a well-established model for the study of neuronal function. Conventional stable isotope labeling with amino acids in...
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Improved Extraction and Detection Method for Bisphenols Using Stable Isotope Labeling Technique
A pair of permanently positively charged stable isotope labeling (SIL) agents,...
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Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trap** for the Detection of Transient Protein Interactions
Antibody-based affinity purification is a recognized method for use in studying protein–protein interactions. There are four different classes of...
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Nitrogen stable isotope analysis of sulfonamides by derivatization-gas chromatography-isotope ratio mass spectrometry
The continuous introduction of micropollutants into the environment through livestock farming, agricultural practices, and wastewater treatment is a...
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Stable Isotope Labeling by Carbon-13 in Bacteria Culture for the Analysis of Residual Avermectin Using Stable Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry
This study describes the development of a new stable isotope labeling method by carbon-13 in bacteria culture (SILCB) for the analysis of residual...
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Comprehensive characterization of differential glycation in hepatocellular carcinoma using tissue proteomics with stable isotopic labeling
Glycation is a non-enzymatic posttranslational modification coming from the reaction between reducing sugars and free amino groups in proteins, where...
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Earthworm effects on soil biogeochemistry in temperate forests focusing on stable isotope tracing: a review
Earthworms (Oligochaeta) are globally distributed soil-dwelling invertebrates that alter soil properties through feeding, casting, and burrowing...
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Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC)
Sumoylation is a dynamic protein posttranslational modification that contributes to many intracellular pathways, including nucleocytoplasmic...
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A Novel Strategy for the Development of Functional Foods to Improve Energy Metabolism Disorders: Stable Isotope-Resolved Metabolomics
With the change of people’s dietary structures, obesity, diabetes, and other related chronic metabolic diseases characterized by energy metabolism...
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Analysis of estrogens in milk samples using ionic liquid-modified covalent organic framework and stable isotope labeling technique
Estrogens in milk samples were extracted by a new ionic liquid (IL)-modified covalent organic framework (COF), and then analyzed by stable isotope...
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Application of 2H stable isotope labelling methodology and ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry for the metabolite identification of dehydroandrographolide in rats
Dehydroandrographolide (DA), one of the crucial diterpenoids of Andrographis paniculata (Burm.F.) Nees, which has been widely used clinically due to...
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Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes
Brown adipose tissue (BAT) demonstrates extraordinary metabolic capacity. Previous research using conventional radio tracers reveals that BAT can act...
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Quantifying label enrichment from two mass isotopomers increases proteome coverage for in vivo protein turnover using heavy water metabolic labeling
Heavy water metabolic labeling followed by liquid chromatography coupled with mass spectrometry is a powerful high throughput technique for measuring...
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15N metabolic labeling-TMT multiplexing approach to facilitate the quantitation of glycopeptides derived from cell lines
The study of glycoproteomics presents a set of unique challenges, primarily due to the low abundance of glycopeptides and their intricate...
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An isotopic labeling approach linking natural products with biosynthetic gene clusters
Major advances in genome sequencing and large-scale biosynthetic gene cluster (BGC) analysis have prompted an age of natural product discovery driven...
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Region-specific mouse brain ganglioside distribution revealed by an improved isobaric aminoxyTMT labeling strategy with automated data processing
Gangliosides are specialized glycosphingolipids most abundant in the central nervous system. Their complex amphiphilic structure is essential to the...
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Method development for 14C-labeling of IgG antibodies in preparation for clinical trials
ObjectivesCarbon-14 ( 14 C) labeling is a standard technology for tracing molecules and providing their pharmacokinetic profiles. However, its primary...
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Comparing 13C methyl and deuterated methyl isotopic labeling for the quantification of methyl cellulose patterns using mass spectrometry
The methyl substitution along and among the polymer chains of methyl cellulose (MC) is commonly analyzed by ESI-MS after perdeuteromethylation of the...
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A potential primary method for peptide purity analysis by gas chromatography-isotope dilution infrared spectrometry
Here we proposed a method for peptide purity analysis using gas chromatography-isotope dilution infrared spectroscopy. The principle and feasibility...
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Dual stable isotopes enhance lipidomic studies in bacterial model organism Enterococcus faecalis
Dual stable isotope probes of deuterium oxide and 13 C fatty acid were demonstrated to probe the lipid biosynthesis cycle of a Gram-positive bacterium E...
