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Long-Range Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a laboratory technique used to amplify a targeted region of DNA, demarcated by a set of oligonucleotide primers....
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Rapid Cycle and Extreme Polymerase Chain Reaction
Rapid cycle polymerase chain reaction (PCR) amplifies DNA in 10–30 min, while extreme PCR is complete in less than 1 min. These methods do not...
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Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection
Aichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with...
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Detection of the phosphorothioate oligonucleotide fomivirsen using a ligase detection reaction with polymerase chain reaction
This study aimed to develop a simple and sensitive detection method for fomivirsen, a 21-nucleotide phosphorothioate oligonucleotide used as a...
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Hydrogel capsule-based digital quantitative polymerase chain reaction
Droplet digital PCR (ddPCR) is accurate in nucleic acid quantification owing to its linearity and high sensitivity. Amplification of nucleic acid in...
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SF-qPCR: Strand Displacement-Based Fast Quantitative Polymerase Chain Reaction
Nucleic acid testing (NAT) is important for the identification and quantification of specific nucleic acid targets, both DNA and RNA, in life...
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An Ultrasensitive Method Using Polymerase Chain Reaction-Dynamic Light Scattering for Detection of Salmonella in Milk
The development of rapid and effective detection of pathogens is necessary to prevent bacterial infection. Herein, we constructed a novel polymerase...
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Neq2X7: a multi-purpose and open-source fusion DNA polymerase for advanced DNA engineering and diagnostics PCR
Thermostable DNA polymerases, such as Taq isolated from the thermophilic bacterium Thermus aquaticus , enable one-pot exponential DNA amplification...
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A Brief Practical Guide to PCR
Polymerase chain reaction (PCR) has been a powerful molecular biology tool since the mid-1980s. Millions of copies of specific sequence regions of...
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Development and intralaboratory validation of three Arcidae species using a multiplex polymerase chain reaction assay combined with capillary electrophoresis
Recently, various commercial ark shell products were being sold, and in the case of processed foods, the loss of morphological traits makes species...
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Emulsion Polymerase Chain Reaction Coupled with Denaturing Gradient Gel Electrophoresis for Microbial Diversity Studies
Emulsion PCR-DGGE is a molecular biology technique used to amplify and analyze DNA fragments. This technique combines two processes, emulsion PCR and...
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TaqMan-based real-time polymerase chain reaction for the detection of feline chaphamaparvovirus
Feline chaphamaparvovirus (FeChPV) is a new viral strain detected in Chinese Mainland in recent years. The symptoms mainly include diarrhea and...
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Megaprimer-Based PCR to Synthesize Fusion Genes for Cloning
Megaprimer-based polymerase chain reaction (PCR) strategies allow the versatile and fast assembly and amplification of a myriad of tailor-made or...
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Improved PCR by the Use of Disruptors, a New Class of Oligonucleotide Reagents
As a powerful tool, polymerase chain reaction (PCR) has been indispensable and widely used in a large array of applications. In practice, many...
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A Sensitive Nucleic Acid Detection Platform for Foodborne Pathogens Based on CRISPR-Cas13a System Combined with Polymerase Chain Reaction
Foodborne diseases attributable to foodborne pathogens directly impact food safety and public health. This study aimed to establish a nucleic acid...
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Quantitative PCR Technique for Predictive Models
This chapter reports the use of real-time quantitative polymerase chain reaction (qPCR) to quantify Weissella viridescens, a spoilage bacteria of...
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Simultaneous detection of feline parvovirus and feline bocavirus using SYBR Green I-based duplex real-time polymerase chain reaction
Since both feline parvovirus (FPV) and feline bocavirus (FBoV) can cause diarrhea in cats, it is difficult to distinguish them clinically. This study...
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Establishment of SYBR green I-based quantitative real-time polymerase chain reaction for the rapid detection of a novel Chaphamaparvovirus in cats
Feline parvovirus causes infectious diseases, and Chaphamaparvovirus is a novel type of feline parvovirus. The present study aims to establish a...
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Bacteria and Yeast Colony PCR
The bacteria Escherichia coli and the yeast Saccharomyces cerevisiae are currently the two most important organisms in synthetic biology. E. coli is...
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Inverse PCR for Site-Directed Mutagenesis
Inverse PCR is a powerful tool for the rapid introduction of desired mutations at desired positions in a circular double-stranded DNA sequence. In...