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One-Step Immunoassay for the Detection of SARS-CoV-2 Nucleocapsid Protein Using Screened Fv-Antibodies
Fv-antibodies against the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were screened from an Fv-antibody...
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One-step immunoassay based on switching peptides for analyzing ochratoxin A in wines
A one-step immunoassay is presented for the detection of ochratoxin A (OTA) using an antibody complex with switching peptides. Because the switching...
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One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher
One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the...
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Gliadin Detection by Immunoassay
Immunoassays are very sensitive and efficient tests commonly used to identify a specific protein. Examples of applications in the food industry...
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NT-proBNP detection with a one-step magnetic lateral flow channel assay
Point-of-care sensors targeting blood marker analysis must be designed to function with very small volumes since acquiring a blood sample through a...
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Development of alkaline phosphatase-linked single-chain variable fragment fusion proteins for one-step immunodetection of deoxynivalenol in cereals
Deoxynivalenol (DON) is a mycotoxin that widely distributes in various foods and seriously threatens food safety. To minimize the consumers’ dietary...
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Rapid determination of domoic acid in seafood by fluorescence polarization immunoassay using a portable analyzer
Monitoring phycotoxin accumulation in marine products such as edible shellfish is a regulatory requirement in many countries. Therefore, a simple and...
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A compact microfluidic laser-induced fluorescence immunoassay system using avalanche photodiode for rapid detection of alpha-fetoprotein
Alpha-fetoprotein (AFP), commonly employed for early diagnosis of liver cancer, serves as a biomarker for cancer screening and diagnosis. Combining...
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A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum
A chemiluminescent immunoassay for human serum Cystatin C (Cys C) was established using a direct-antibody sandwich model. The immunoassay kit uses...
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Microgel-based etalon immunoassay for IgG detection
We developed an immunoassay for mouse immunoglobulin (IgG) quantitation using poly( N -isopropylacrylamide- co -acrylic acid) (pNIPAm- co -AAc)...
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Nanobody based immunoassay for alpha fetal protein detection using streptavidin-conjugated polymerized horseradish peroxidase for signal amplification
AbstractThe enzyme-linked immunosorbent assay (ELISA) offers several advantages, including simple operation, high throughput, and low cost, making it...
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Development of quantitative detection methods for four Alzheimer’s disease specific biomarker panels using electrochemical immunosensors based on enzyme immunoassay
Accurate and timely diagnosis of Alzheimer's disease (AD) is necessary to maximize the effectiveness of treatment and using biomarkers for diagnosis...
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Biomimetic nanochannels for molybdate transport: application to sensitive electrochemical immunoassay for HER2
A nanochannel-based electrochemical immunoassay was developed for the detection of human epidermal growth factor receptor 2 (HER2), with molybdate as...
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Microfluidic chip immunoassay based on rolling circle amplification and G-quadruplex/Thioflavin T for multiplex detection of CTX I
A label-free immunoassay based on rolling circle amplification (RCA) and G-quadruplex/Thioflavin T (G4/ThT) is proposed to realize the sensitive...
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Establishment of Sensitive Sandwich-Type Chemiluminescence Immunoassay for Interleukin-18 in Urinary Samples
Interleukin-18 (IL-18), a member of IL-1 cytokine superfamily, is deemed as an important indicator of the kidney disease. Herein a sandwich...
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Fluorescence ratio immunoassay for fumonisin B1 based on the oxidase characteristics of the growth of monodispersed 2-D MnO2 nanosheet on an individual gold nanoparticle (AuNP@MnO2)
Fumonisin B1 (FB1) is one of the important mycotoxins posing health risks in the area of food safety. A sensitive fluorescence ratio immunoassay has...
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Simultaneous Triplex Detection in a Single-Test-Line Lateral Flow Immunoassay Utilizing Distinct Nanoparticle Colorimetry
Lateral flow immunoassay (LFIA) has become a popular method for the rapid detection of biological molecules, with an emerging need for multiplex...
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Glycylglycine promotes the solubility and antigenic utility of recombinant HCV structural proteins in a point-of-care immunoassay for detection of active viremia
BackgroundAlthough E. coli is generally a well-opted platform for the overproduction of recombinant antigens as heterologous proteins, the...
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A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the...
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Using Carbon Quantum Dots for the Determination of Aminoglycoside Antibiotics by Fluorescence Polarization Immunoassay
AbstractConditions are studied for the synthesis of carbon quantum dots (CQD) exhibiting green fluorescence by the ultrasonic treatment of...
