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Improving the catalytic characteristics of phenolic acid decarboxylase from Bacillus amyloliquefaciens by the engineering of N-terminus and C-terminus
Background4-vinylphenols produced by phenolic acid degradation catalyzed by phenolic acid decarboxylase can be used in food additives as well as...
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Diselenide-bond replacement of the external disulfide bond of insulin increases its oligomerization leading to sustained activity
Seleno-insulin, a class of artificial insulin analogs, in which one of the three disulfide-bonds (S-S’s) of wild-type insulin (Ins) is replaced by a...
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Organometallic Receptors for Biologically Interesting Molecules
Trinuclear organometallic macrocycles are able to complex amino acids and peptides in aqueous solution. The complexation, which is driven by...
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Non-modular fatty acid synthases yield distinct N-terminal acylation in ribosomal peptides
Recent efforts in genome mining of ribosomally synthesized and post-translationally modified peptides (RiPPs) have expanded the diversity of...
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Structure and mechanism of a neuropeptide-activated channel in the ENaC/DEG superfamily
Phe-Met-Arg-Phe-amide (FMRFamide)-activated sodium channels (FaNaCs) are a family of channels activated by the neuropeptide FMRFamide, and, to date,...
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Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
BackgroundThe filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading...
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Investigating the Effect of Substituting a Single Cysteine Residue on the Thermal Stability of an Engineered Sweet Protein, Single-Chain Monellin
Single-chain monellin (SCM) is an engineered protein that links the two chains of monellin, a naturally sweet-tasting protein. This protein is an...
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Complete Model of Vinculin Suggests the Mechanism of Activation by Helical Super-Bundle Unfurling
To shed light onto the activation mechanism of vinculin, we carried out a detailed refinement of chicken vinculin and compared it to the human...
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N-terminal domain replacement changes an archaeal monoacylglycerol lipase into a triacylglycerol lipase
BackgroundLipolytic enzymes of hyperthermophilic archaea generally prefer small carbon chain fatty acid esters (C 2 –C 12 ) and are categorized as...
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Efficient production and biochemical characterization of a thermostable carboxypeptidase from Bacillus megaterium and its application on flavor improvement of soy isolate protein hydrolysates
A carboxypeptidase M32 (CPM32) gene ( cpm32 ) from Bacillus megaterium was cloned. CPM32 exhibited the highest sequence similarity of 79.3% with the...
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Engineering a carbohydrate-binding module to increase the expression level of glucoamylase in Pichia pastoris
BackgroundGlucoamylase is an important industrial enzyme for the saccharification of starch during sugar production, but the production cost of...
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Engineering Homogeneous Photoactive Antibody Fragments
Genetically encoded site-specifically incorporated noncanonical amino acids (ncAAs) have been used to modulate properties of several proteins. Here,...
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Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae
The expression of plant cysteine oxidase (PCO) enzyme in Saccharomyces cerevisiae enables the Arg/Cys N-degron pathway (Cys-NDP) for selective...
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Identification and determination of structurally related peptide impurities in thymalfasin by liquid chromatography-high-resolution mass spectrometry
Thymalfasin is an important peptide drug widely used for the single or combination treatment of hepatitis, sepsis, cancer, and immunodeficiency....
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Screening and Constructing of Novel Angiotensin I-Converting Enzyme Inhibiting Peptides from Walnut Protein Isolate and Their Mechanisms of Action: A Merged In Silico and In Vitro Study
Angiotensin I-converting enzyme (ACE)-inhibiting peptides were isolated from walnut protein isolate (WPI) using ultrasound-assisted extraction. This...
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Regulation of Gene Expression by Synthetic DNA-Binding Ligands
During the past 20 years, polyamides have evolved from the natural product distamycin to a new class of programmable heterocyclic oligomers that bind...
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New Organometallic Technetium Complexes for Radiopharmaceutical Imaging
It is the objective of this review to introduce the reader to the current status of organometallic chemistry, oriented towards the development of...
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Light-driven biosynthesis of volatile, unstable and photosensitive chemicals from CO2
Directing CO 2 conversion using photoautotrophic microbes offers a promising route to coupling carbon mitigation with petrochemical replacement....
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A new halotolerant xylanase from Aspergillus clavatus expressed in Escherichia coli with catalytic efficiency improved by site-directed mutagenesis
Daily agro-industrial waste, primarily cellulose, lignin, and hemicellulose, poses a significant environmental challenge. Harnessing...
