Search
Search Results
-
Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae
Microbial proteins are promising substitutes for animal- and plant-based proteins. S. cerevisiae , a generally recognized as safe (GRAS)...
-
Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
BackgroundThe filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading...
-
Heterologous Production of Antimicrobial Peptides: Notes to Consider
Heavy and irresponsible use of antibiotics in the last century has put selection pressure on the microbes to evolve even faster and develop more...
-
Understanding the Necessity of Regulatory Protein Machinery in Heterologous Expression of Class-III Type of Ocins
To date, there have been no or just a few reports of successful cloning and expression to create biologically active ocins or bacteriocins. Cloning,...
-
A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli
BackgroundProteins with novel functions or advanced activities developed by various protein engineering techniques must have sufficient solubility to...
-
Heterologous Expression of Toxic White Spot Syndrome Virus (WSSV) Protein in Eengineered Escherichia coli Strains
Aquacultural shrimps suffer economic lost due to the white spot syndrome virus (WSSV) that is the most notorious virus for its fatality and...
-
“Metabolic burden” explained: stress symptoms and its related responses induced by (over)expression of (heterologous) proteins in Escherichia coli
BackgroundEngineering bacterial strains to redirect the metabolism towards the production of a specific product has enabled the development of...
-
The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis
Anthocyanins are ubiquitous plant pigments used in a variety of technological applications. Yet, after over a century of research, the penultimate...
-
Tobacco Plant: A Novel and Promising Heterologous Bioreactor for the Production of Recombinant Bovine Chymosin
The natural source of chymosin, a key enzyme in the dairy industry, is insufficient for rapidly growing cheese industries. Large-scale production of...
-
Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
BackgroundPichia pastoris (syn. Komagataella phaffii ) is an important yeast system for heterologous protein expression. A robust P. pastoris mutant...
-
Engineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coli
BackgroundLipoxygenase (EC. 1.13.11.12, LOX) can catalyze the addition of oxygen into polyunsaturated fatty acids to produce hydroperoxides, which...
-
Development of wheat bran hydrolysate as Komagataella phaffii medium for heterologous protein production
Develo** a Komagataella phaffii ( K. phaffii, named as Pichia pastoris formerly) medium using wheat bran hydrolysate (WBH) is a potential approach...
-
Heterologous production of the insecticidal pea seed albumin PA1 protein by Pichia pastoris and protein engineering to potentiate aphicidal activity via fusion to snowdrop lectin Galanthus nivalis agglutinin; GNA)
BackgroundNew bioinsecticides with novel modes of action are urgently needed to minimise the environmental and safety hazards associated with the use...
-
Plant Platforms for Efficient Heterologous Protein Production
Production of recombinant proteins is primarily established in cultures of mammalian, insect and bacterial cells. Concurrently, concept of using...
-
Application of an Autoinduction Strategy to Optimize the Heterologous Production of an Antitumor Bispecific Fusion Protein Based on the TRAIL Receptor-Selective Mutant Variant in Escherichia coli
Autoinduction is a simple approach for heterologous protein expression that helps to achieve the high-level production of recombinant proteins in...
-
Heterologous overproduction of oviedomycin by refactoring biosynthetic gene cluster and metabolic engineering of host strain Streptomyces coelicolor
BackgroundOviedomycin is one among several polyketides known for their potential as anticancer agents. The biosynthetic gene cluster (BGC) for...
-
Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910
Unspecific peroxygenases (UPOs) are glycosylated enzymes that provide an efficient method for oxyfunctionalizing a variety of substrates using only...
-
Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering
BackgroundMost of the proteases classified into the M23 family in the MEROPS database exhibit staphylolytic activity and have potential as...
-
Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry
BackgroundThe wasabi receptor, also known as the Transient Receptor Potential Ankyrin 1 (TRPA1) ion channel, is a potential target for development of...
-
Expression of heterologous heparan sulphate binding protein of Helicobacter pylori on the surface of Lactobacillus rhamnosus GG
Helicobacter pylori ( H. pylori ) is one of most commonly found pathogen in the stomach. In spite of emergence of different treatment strategies, H....
