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Transistor-based immunosensor using AuNPs-Ab2-HRP enzyme nanoprobe for the detection of antigen biomarker in human blood
Alpha-fetoprotein (AFP) is inextricably linked to various diseases, including liver cancer. Thus, detecting the content of AFP in biology has great...
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A Smartphone Colorimetric Development with TMB/H2O2/HRP Reaction System for Hydrogen Peroxide Detection and its Applications
AbstractThe residue and overproduction of hydrogen peroxide (H 2 O 2 ) can harm cellular dysfunction under oxidative stress conditions. The development...
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Visual dual-mode aptasensor for non-small cell lung cancer exosome detection via HRP self-coupling enhanced oxidized iridium nanoparticle aggregation
The main reason for the high mortality rate of non-small cell lung cancer is that patients are usually diagnosed at an advanced stage of the disease....
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Engineering a thermostable biosensor based on biomimetic mineralization HRP@Fe-MOF for Alzheimer’s disease
The development of disease detection by biosensors represents one of the key components of medical science. However, millions of people are still...
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Electrochemical sensing platform for detection of heavy metal ions without electrochemical signal
Heavy metal pollution has attracted global attention because of its high toxicity, non-biodegradability, and carcinogenicity. Electrochemical sensors...
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A colorimetric, photothermal, and fluorescent triple-mode CRISPR/cas biosensor for drug-resistance bacteria detection
A multimodal analytical strategy utilizing different modalities to cross-validate each other, can effectively minimize false positives or negatives...
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A versatile and ultrasensitive molecularly imprinted electrochemiluminescence sensor with HRP-encapsulated liposome labeled by light-triggered click reaction for pesticide residues
A novel approach for trace detection of fipronil with a molecularly imprinted electrochemiluminescence sensor (MIECLS) is proposed. The sensitivity...
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Sandwich-type microRNA biosensor based on graphene oxide incorporated 3D-flower-like MoS2 and AuNPs coupling with HRP enzyme signal amplification
A sandwich electrochemical biosensing strategy for ultrasensitive detection of miRNA-21 was developed by using graphene oxide incorporated...
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Dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase-tethered magnetic microspheres for colorimetric detection of microcystin-LR
A novel dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase (HRP) was developed for colorimetric determination of MC-LR. This...
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An Antibody Ordered Assembly Functional BPE-ECL Platform for Aflatoxin B1 Detection
Due to the complex characteristics of food matrix, false positives may occur when using traditional methods to detect aflatoxin B1 (AFB1). In this...
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A sensitive, accurate, and high-throughput gluco-oligosaccharide oxidase-based HRP colorimetric method for assaying lytic polysaccharide monooxygenase activity
BackgroundThe AA9 (auxiliary activities) family of lytic polysaccharide monooxygenases (AA9 LPMOs) is a ubiquitous and diverse group of enzymes in...
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A Novel Enzyme-Based Electrochemical Biosensor for Sensitive Detection of Hydrogen Peroxide Based on the Fluorescent Peptide Self-Assembled Nanomaterials
Advancing enzyme-based electrochemical biosensor research heightens the need for improved biosafety and reduced biotoxicity. Conventional electrode...
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Ultrasensitive colorimetric detection of Staphylococcus aureus using wheat germ agglutinin and IgY as a dual-recognition strategy
A novel colorimetric platform was designed for the determination of S. aureus by utilizing a dual-recognition strategy, where wheat germ agglutinin...
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An integrated magnetic separation enzyme-linked colorimetric sensing platform for field detection of Escherichia coli O157: H7 in food
An intelligent colorimetric sensing platform integrated with in situ immunomagnetic separation function was developed for ultrasensitive detection of E...
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Microgel-based etalon immunoassay for IgG detection
We developed an immunoassay for mouse immunoglobulin (IgG) quantitation using poly( N -isopropylacrylamide- co -acrylic acid) (pNIPAm- co -AAc)...
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Multiorbital DNA walker nanoprobe for portable photothermal detection based on H2S etching of cubic Prussian blue
In the developed assay, multiorbital 3D DNA walker (MO DNA walker) was applied as signal amplified protocol for enhancing the detection signal of the...
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Detection of SARS-CoV-2 receptor binding domain using fluorescence probe and DNA flowers enabled by rolling circle amplification
Using rolling circle amplification (RCA) and two different ways of signal readout, we developed analytical methods to detect the receptor-binding...
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Nanobody based immunoassay for alpha fetal protein detection using streptavidin-conjugated polymerized horseradish peroxidase for signal amplification
AbstractThe enzyme-linked immunosorbent assay (ELISA) offers several advantages, including simple operation, high throughput, and low cost, making it...
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High-Throughput Bioassay for Detection of Latent Fungi in Postharvest Produce
Enormous fresh agricultural produce is wasted annually due to rots caused by pathogenic microorganisms. Most pathogenic fungi attack the harvested...
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Electrochemiluminescent immunosensor for detection of carcinoembryonic antigen using luminol-coated silver nanoparticles
Recently, electrochemiluminescent (ECL) immunosensors have received much attention in the field of biomarker detection. Here, a highly enhanced ECL...