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Positive Selection Screens for Programmable Endonuclease Activity Using I-SceI
Positive selection screens are high-throughput assays to characterize novel enzymes from environmental samples and enrich for more powerful variants...
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A High-Affinity Methyl-CpG-Binding Protein for Endonuclease-Free and Label-Free DNA Methyltransferase Activity Detection
DNA methyltransferase (MTase) activity detection has received increasing attention as a promising biomarker and therapeutic target. However, most of...
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T790M mutation upconversion fluorescence biosensor via mild ATRP strategy and site-specific DNA cleavage of restriction endonuclease
A unique combination of a specific nucleic acid restriction endonuclease (REase) and atom transfer radical polymerization (ATRP) signal amplification...
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Rapid and ultrasensitive miRNA detection by combining endonuclease reactions in a rolling circle amplification (RCA)–based hairpin DNA fluorescent assay
MicroRNA (miRNA) sensing strategies employing rolling circle amplification (RCA) coupled with the hairpin DNA (HD) probe–mediated FRET assay have...
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T7 Endonuclease I-mediated voltammetric detection of KRAS mutation coupled with horseradish peroxidase for signal amplification
Rapid and selective sensing of KRAS gene mutation which plays a crucial role in the development of colorectal, pancreatic, and lung cancers is of...
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Dual colorimetric strategy for specific DNA detection by nicking endonuclease-assisted gold nanoparticle signal amplification
The continuous spread of invasive alien species, as zebra mussel ( Dreissena polymorpha ), is a major global concern and it is urgent to stop it. Early...
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Electrochemiluminescence biosensor for determination of lead(II) ions using signal amplification by Au@SiO2 and tripropylamine-endonuclease assisted cycling process
MXene@Au as the base and Au@SiO 2 as signal amplification factor were used for constructing an ultrasensitive “on–off” electrochemiluminescence (ECL)...
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Argonaute with stepwise endonuclease activity promotes specific and multiplex nucleic acid detection
Argonaute proteins (Agos) from thermophiles function as endonucleases via guide-target base-pairing cleavage for host defense. Since guides play a...
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Homogeneous photoelectrochemical biosensor for microRNA based on target-responsive hydrogel coupled with exonuclease III and nicking endonuclease Nb.BbvCI assistant cascaded amplification strategy
MicroRNAs can serve as biomarkers for many cancers, so it is significant to develop simple and sensitive strategies for microRNAs detection....
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Lung cancer: progression of heat shock protein 70 in association with flap endonuclease 1 protein
Lung cancer is one of the leading causes of cancer deaths worldwide and existing approaches are not enough to manage, and hence, it is important to...
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Fluorescence determination of the activity of O6-methylguanine-DNA methyltransferase based on the activation of restriction endonuclease and the use of graphene oxide
A fluorescence method is described for the determination of the activity of O 6 -methylguanine-DNA methyltransferase (MGMT). It is based on the...
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An innovative molecular approach towards the cost-effective entomological authentication of honey
Honey authentication and traceability are crucial not only for economic purposes but also for ensuring safety. However, the widespread adoption of...
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Variety Differentiation: Development of a CRISPR DETECTR Method for the Detection of Single Nucleotide Polymorphisms (SNPs) in Cacao (Theobroma cacao) and Almonds (Prunus dulcis)
To prevent food fraud, products can be monitored by various chemical-analytical techniques. In this study, we present a CRISPR-Cpf1 DETECTR-based...
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DNA cleavage by endonuclease I-DmoI: a QM/MM study and comparison with experimental data provide indications on the environmental effects
Here, we present the theoretical–computational modelling of the free energy barrier of the phosphodiester bond cleavage as occurring in the...
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DNA Damage and Repair in G-Quadruplexes Impact Gene Expression
Guanine-rich sequences in DNA are sensitive to oxidation to 8-oxo-7,8-dihydroguanine (OG) during cellular oxidative stress. When this base...
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DNA Damage and Repair in G-Quadruplexes Impact Gene Expression
Guanine-rich sequences in DNA are sensitive to oxidation to 8-oxo-7,8-dihydroguanine (OG) during cellular oxidative stress. When this base...
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Dual signal amplification coupling with DNA-templated silver nanoclusters for sensitive and label-free detection of thrombin
Sensitive and reliable determination of thrombin is relevant in the realms of medical and biological research as it serves as an essential biomarker...
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Evolution of endonuclease IV protein family: an in silico analysis
DNA repair is one of the key cellular events which balances between evolvability and integrity of the genome. Endonuclease IV enzymes are class II AP...
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CRISPR/Cas9 System: An Advanced Approach for the Improvement of Industrially Important Microorganisms
Genetic engineering is a complex process that refines genes at the molecular level. The field of industrial biotechnology relies much on genetic...
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Assessing and engineering the IscB–ωRNA system for programmed genome editing
OMEGA RNA (ωRNA)-guided endonuclease IscB, the evolutionary ancestor of Cas9, is an attractive system for in vivo genome editing because of its...
