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Simultaneous detection of feline parvovirus and feline bocavirus using SYBR Green I-based duplex real-time polymerase chain reaction
Since both feline parvovirus (FPV) and feline bocavirus (FBoV) can cause diarrhea in cats, it is difficult to distinguish them clinically. This study...
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Ultra-sensitive electrochemiluminescent biosensor for miRNA based on CRISPR/Cas13a trans-cleavage-triggered hybridization chain reaction and magnetic-assisted enrichment
The great selectivity and trans-cleavage activity of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a had been coupled with...
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Duplex droplet digital PCR (ddPCR) for simultaneous quantification of bovine and porcine gelatin in capsules
Detection of bovine and porcine in gelatin-based products is important as species fraud and product mislabeling may have a detrimental impact on...
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Recombinase Polymerase and Loop-Mediated Isothermal Amplification in the DNA Diagnostics of Infectious Diseases
AbstractRecombinase polymerase and loop-mediated isothermal amplification can be conducted in nonlaboratory conditions, making these methods...
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Development of surface-enhanced Raman scattering-sensing Method by combining novel Ag@Au core/shell nanoparticle-based SERS probe with hybridization chain reaction for high-sensitive detection of hepatitis C virus nucleic acid
The ultrasensitive detection of hepatitis C virus (HCV) nucleic acid is crucial for the early diagnosis of hepatitis C. In this study, by combining...
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Identification of DNA variants at ultra-low variant allele frequencies via Taq polymerase cleavage of wild-specific blockers
Detecting mutations related to tumors holds immense clinical significance for cancer diagnosis and treatment. However, the presence of highly...
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Electrochemical biosensor for detecting pathogenic bacteria based on a hybridization chain reaction and CRISPR-Cas12a
In this study, Lba Cas12a (Cpf1) as one of the CRISPR systems from Lachnospiraceae bacterium was coupled with a hybridization chain reaction (HCR) to...
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Exonuclease III assisted exponential amplification reaction (EXPAR) for specific miRNA-155 analysis during post-anesthetic nursing
The persistent obstacle in precise and sensitive identification of microRNAs (miRNAs) pertains to the advancement of expeditious and effective...
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Label-free and sensitive MiRNA detection based on turn-on fluorescence of DNA-templated silver nanoclusters coupled with duplex-specific nuclease-assisted signal amplification
AbstractA novel strategy for microRNAs (miRNAs) detection has been developed utilizing duplex-specific nuclease-assisted signal amplification...
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Ratiometric electrochemical detection of miRNA based on DNA nanomachines and strand displacement reaction
MicroRNAs (miRNAs) play an important role in regulating gene expression in cells. Abnormal expression of miRNAs has been associated with a variety of...
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Assessment of the Real-Time PCR Method Claiming to be Specific for Detection and Quantification of the First Commercialised Genome-Edited Plant
A real-time PCR method was recently published with a claim to be specific for the detection and identification of some genome-edited oilseed rape...
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Ultrasensitive Reverse Transcriptase Loop-Mediated Isothermal Amplification (US-LAMP)-Based Detection of Malaria Infection from Dried Blood Spots
Submicroscopic malaria diagnosis requires highly sensitive tools instead of the conventional microscopy and rapid diagnostic tests (RDTs). While...
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Meat Species Identification: Amplification Refractory Mutation System-Polymerase Chain Reaction–Based Assay
True verification of meat species is critical for religious, economical, legal, and/or public health concerns. Current methods available for...
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Reference material development for detection of human respiratory syncytial virus using digital PCR
Nucleic acid testing is a powerful tool for the detection of various pathogens. Respiratory syncytial virus (RSV) is a major cause of acute...
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Improved PCR by the Use of Disruptors, a New Class of Oligonucleotide Reagents
As a powerful tool, polymerase chain reaction (PCR) has been indispensable and widely used in a large array of applications. In practice, many...
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An Efficient Droplet Digital PCR Approach for Detection DNA at Low Concentrations of Toxigenic Fungi in Food Products
As common toxigenic fungi genera, Aspergillus , Penicillium , and Fusarium , have attracted the attention of governments and people all over the world...
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A simple and label-free proximity-catalytic hairpin reaction-based method for sensitive and miRNA analysis
Aberrant expression of microRNAs (miRNAs) has been shown to be linked to several crucial biological processes, including as carcinogenesis,...
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Target amplification-free detection of urinary microRNA for diabetic nephropathy diagnosis with electrocatalytic reaction
Diabetic nephropathy (DN) is a serious diabetic complication, usually developed from type II diabetes mellitus (T2DM) and known as type II DN (T2DN)....
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Rapid and simultaneous visual ty** of high-risk HPV-16/18 with use of integrated lateral flow strip platform
A biosensor for rapid and simultaneous visual identification of high-risk human papillomavirus (HPV) genotypes 16 and 18 in clinical samples based on...
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Dry reagent-based multiplex real-time PCR assays for specific identification of chicken, mutton, beef and pork in raw and processed meat products
DNA-based methods are more reliable than the conventional protein-based techniques and have been applied to species identification in food of animal...
