Search
Search Results
-
Formulation of Chitosan–Zein Nano-in-Microparticles for Oral DNA Delivery
Gene delivery via the oral route offers a promising strategy for improving DNA vaccination and gene-based therapy outcomes. The noninvasive nature of...
-
DNA-Based Hydrogel in Drug Delivery
DNA is perhaps the most important biopolymer that stores genetic information and is an integrated part of most living cells. Due to its high water...
-
Berberine Degradation Characteristics and its Degradation Pathway by a Newly Isolated Berberine-Utilizing Agrobacterium
Berberine (BBR) is widely used as a botanical pesticide due to its broad-spectrum antibacterial and antifungal activities. However, BBR degradation...
-
Enzymatic Degradation of Capsules Based on Polyelectrolyte Polypeptide–Polysaccharide Complex for the Controlled Release of DNA
AbstractDNA-containing biodegradable microcapsules based on vaterite CaCO 3 polycrystalline particles and polyelectrolyte complex...
-
DNA Adsorption on Carbonaceous Materials
The immobilization of DNA on different solid supports has become an important issue in different fields ranging from medicine to analytical...
-
Optimization of Microbial DNA Extraction from Wine, Juice, and Sap for Community-Based Genome Studies
Obtaining high-quality DNA for diversity analysis from plant-based samples is challenging due to the presence of inhibitors, such as phenolics which...
-
UFL1 triggers replication fork degradation by MRE11 in BRCA1/2-deficient cells
The stabilization of stalled forks has emerged as a crucial mechanism driving resistance to poly(ADP-ribose) polymerase (PARP) inhibitors in...
-
DNA Sizing with pg/μL Sensitivity for Cell-Free DNA Analysis with the μLAS Technology
The μLAS technology enables in-line DNA concentration and separation in a microchannel. Here, we describe its operation to analyze the size profile...
-
DNA Immobilization: Silanized Nucleic Acids and Nanoprinting
Over the last decade, the development of DNA microarray technology has allowed the simultaneous analysis of many thousands of different genes in...
-
A CRISPR activation screen identifies FBXO22 supporting targeted protein degradation
Targeted protein degradation (TPD) represents a potent chemical biology paradigm that leverages the cellular degradation machinery to...
-
Assessment of Degradation of Mucin
Mucin degradation is an important safety aspect for the identification of probiotics bacteria, as excessive mucin degradation cause damage to...
-
DNA-encoded library-enabled discovery of proximity-inducing small molecules
Small molecules that induce protein–protein associations represent powerful tools to modulate cell circuitry. We sought to develop a platform for the...
-
Bisulfite-free map** of DNA cytosine modifications: challenges and perspectives
The map** of DNA cytosine modifications is crucial for understanding the dynamic landscape of epigenetic regulation. While bisulfite sequencing has...
-
Assaying ATE1 Activity in Yeast by β-Gal Degradation
In the 1980s, it was found that addition of N-terminal Arg to proteins induces their ubiquitination and degradation by the N-end rule pathway. While...
-
DNA Knots
Knotting is statistically inevitable in long polymer chains, especially under spatial confinement, and tightly packed genomic DNA filaments are no...
-
Targeted degradation via direct 26S proteasome recruitment
Engineered destruction of target proteins by recruitment to the cell’s degradation machinery has emerged as a promising strategy in drug discovery....
-
dCas12a:Pre-crRNA: A New Tool to Induce mRNA Degradation in Saccharomyces cerevisiae Synthetic Gene Circuits
We describe a new way to trigger mRNA degradation in Saccharomyces cerevisiae synthetic gene circuits. Our method demands to modify either the 5′- or...
-
Targeting DNA with Triplexes
Triple helical nucleic acids are generated by the binding of a third oligonucleotide strand within the major groove of duplex DNA or RNA, where it...
-
Targeting DNA with Triplexes
Triple helical nucleic acids are generated by the binding of a third oligonucleotide strand within the major groove of duplex DNA or RNA, where it...
-
Biomaterials Based on DNA Conjugates and Assemblies
In this chapter, biomaterial development based on DNA-sugar and DNA-peptide conjugates and DNA assemblies is highlighted. The conjugation of sugar...