Search
Search Results
-
Optimizing yeast for high-level production of kaempferol and quercetin
BackgroundTwo important flavonoids, kaempferol and quercetin possess remarkably potent biological impacts on human health. However, their structural...
-
Optimization of the production process for the anticancer lead compound illudin M: process development in stirred tank bioreactors
BackgroundThe fungal natural products illudin S and M have been investigated as precursors for the development of semisynthetic anticancer agents...
-
Metabolic engineering of yeast for the production of carbohydrate-derived foods and chemicals from C1–3 molecules
The increase in population-related and environmental issues has emphasized the need for more efficient and sustainable production strategies for...
-
Itaconate Production from Crude Substrates with U. maydis: Scale-up of an Industrially Relevant Bioprocess
BackgroundIndustrial by-products accrue in most agricultural or food-related production processes, but additional value chains have already been...
-
High-efficiency production of 5-hydroxyectoine using metabolically engineered Corynebacterium glutamicum
BackgroundExtremolytes enable microbes to withstand even the most extreme conditions in nature. Due to their unique protective properties, the small...
-
High-throughput process development from gene cloning to protein production
In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In...
-
Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing
BackgroundThe abundance of glucuronoxylan (GX) in agricultural and forestry residual side streams positions it as a promising feedstock for microbial...
-
First time β-farnesene production by the versatile bacterium Cupriavidus necator
BackgroundTerpenes are remarkably diverse natural structures, which can be formed via two different pathways leading to two common intermediates....
-
From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pastoris
BackgroundExpansion of the genetic code is a frequently employed approach for the modification of recombinant protein properties. It involves...
-
Parallel Reactor Systems for Bioprocess Development
Controlled parallel bioreactor systems allow fed-batch operation at early stages of process development. The characteristics of shaken bioreactors...
-
Control of parallelized bioreactors II: probabilistic quantification of carboxylic acid reductase activity for bioprocess optimization
Autonomously operated parallelized mL-scale bioreactors are considered the key to reduce bioprocess development cost and time. However, their...
-
A production platform for disulfide-bonded peptides in the periplasm of Escherichia coli
BackgroundRecombinant peptide production in Escherichia coli provides a sustainable alternative to environmentally harmful and size-limited chemical...
-
High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions
BackgroundPediocin PA-1 is a bacteriocin of recognized value with applications in food bio-preservation and the medical sector for the prevention of...
-
Upgrading dilute ethanol to odd-chain carboxylic acids by a synthetic co-culture of Anaerotignum neopropionicum and Clostridium kluyveri
BackgroundDilute ethanol streams generated during fermentation of biomass or syngas can be used as feedstocks for the production of higher value...
-
Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
BackgroundThe split GFP assay is a well-known technology for activity-independent screening of target proteins. A superfolder GFP is split into two...
-
Biotechnological potential and initial characterization of two novel sesquiterpene synthases from Basidiomycota Coniophora puteana for heterologous production of δ-cadinol
BackgroundTerpene synthases are versatile catalysts in all domains of life, catalyzing the formation of an enormous variety of different terpenoid...
-
Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli
BackgroundEscherichia coli is one of the most commonly used host organisms for the production of biopharmaceuticals, as it allows for cost-efficient...
-
Engineering co-utilization of glucose and xylose for chemical overproduction from lignocellulose
Bio-refining lignocellulose could provide a sustainable supply of fuels and fine chemicals; however, the challenges associated with the...
-
Whole cell-based catalyst for enzymatic production of the osmolyte 2-O-α-glucosylglycerol
BackgroundGlucosylglycerol (2- O -α- d -glucosyl- sn -glycerol; GG) is a natural osmolyte from bacteria and plants. It has promising applications as...
-
Control of parallelized bioreactors I: dynamic scheduling software for efficient bioprocess management in high-throughput systems
The shift towards high-throughput technologies and automation in research and development in industrial biotechnology is highlighting the need for...
