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In silico evaluation of a targeted metaproteomics strategy for broad screening of cellulolytic enzyme capacities in anaerobic microbiome bioreactors
BackgroundMicrobial-driven solubilization of lignocellulosic material is a natural mechanism that is exploited in anaerobic digesters (ADs) to...
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Substantial defluorination of polychlorofluorocarboxylic acids triggered by anaerobic microbial hydrolytic dechlorination
Chlorinated polyfluorocarboxylic acids (Cl-PFCAs) derived from the widely used chlorotrifluoroethylene oligomers and polymers may enter and influence...
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Cultivation Techniques and Molecular Methods of Identification of Thermophilic, Anaerobic Bacteria
The cultivation and identification of strictly anaerobic thermophilic microorganisms present a number of challenges owing to the oxygen-sensitive...
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Biodegradability of Biodegradable Plastics in Compost, Marine, and Anaerobic Environments Assessed by Automated Respirometry
Biodegradability is an increasingly beneficial property of sustainable materials, particularly for single-use packaging. Biodegradation rates can...
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Lutein Production by Halophilic Microalgae Using Anaerobic Digestate as the Substrate and Its Potential Application as a Biopesticide
Production of value-added products from waste anaerobic digestate is economically and environmentally important for sustainable development of...
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Copper sulfide mineral performs non-enzymatic anaerobic ammonium oxidation through a hydrazine intermediate
Anaerobic ammonium oxidation (anammox)—the biological process that activates ammonium with nitrite—is responsible for a significant fraction of N 2 ...
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Screening for New Metabolites from Marine Microorganisms
This article gives an overview of current analysis techniques for the screening and the activity analysis of metabolites from marine...
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CRISPRi-Driven Genetic Screening for Designing Novel Microbial Phenotypes
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has enabled rapid advances in genomic engineering and transcriptional...
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Improvement of 1,3-propanediol production from crude glycerol by co-cultivation of anaerobic and facultative microbes under non-strictly anaerobic conditions
BackgroundNatural microbial consortia could efficiently produce 1,3-propanediol (1,3-PDO), a most promising bulk biochemical derived from glycerol...
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Different stages of microbial community during the anaerobic digestion of food waste
Large-scale food waste (FW) disposal has resulted in severe environmental degradation and financial losses around the world. Although FW has a high...
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Performance of Paracoccus pantotrophus MA3 in heterotrophic nitrification–anaerobic denitrification using formic acid as a carbon source
Excess amount of nitrogen in wastewater has caused serious concerns, such as water eutrophication. Paracoccus pantotrophus MA3, a novel isolated...
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Current challenges of high-solid anaerobic digestion and possible measures for its effective applications: a review
The attention that high solids anaerobic digestion process (HS-AD) has received over the years, as a waste management and energy recovery process...
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Evaluation of microtiter plate as a high-throughput screening platform for beer fermentation
Downscaling the anaerobic fermentation in a microtiter plate (MTP) facilitates high throughput screening (HTS) applications. This study investigates...
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Automation assisted anaerobic phenoty** for metabolic engineering
BackgroundMicroorganisms can be metabolically engineered to produce a wide range of commercially important chemicals. Advancements in computational...
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Galactose-based biohydrogen production from seaweed biomass by novel strain Clostridium sp. JH03 from anaerobic digester sludge
Seaweed biomass in Korea is rich in galactose following hydrolysis, and leveraging this resource for enhancing the biohydrogen production is the aim...
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New high-throughput screening method for Chinese hamster ovary cell lines expressing low reduced monoclonal antibody levels: application of a system controlling the gas phase over cell lysates in miniature bioreactors and facilitating multiple sample setup
Interchain disulfide bonds in monoclonal antibodies may be reduced during large-scale mAb production using Chinese hamster ovary (CHO) cells. This...
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QPCR-Guided Screening of Levansucrase: Levan Characterization and Genomic Insights
Eighty-seven bacteria isolates were screened for the presence of the levansucrase encoding gene and those possessing the gene were used in gene...
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Biogas and Volatile Fatty Acid Production During Anaerobic Digestion of Straw, Cellulose, and Hemicellulose with Analysis of Microbial Communities and Functions
The anaerobic digestion efficiency and methane production of straw was limited by its complex composition and structure. In this study, rice straw...
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Development of two devices for high-throughput screening of ethanol-producing microorganisms by real-time CO2 production monitoring
Bioethanol’s importance as a renewable energy carrier led to the development of new devices for the high-throughput screening (HTS) of...
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Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum
BackgroundThe intracellular ATP level is an indicator of cellular energy state and plays a critical role in regulating cellular metabolism. Depletion...
