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Thermotolerant and protease-resistant GH5 family β-mannanase with CBM1 from Penicillium aculeatum APS1: purification and characterization
In past several years, mannanases has attracted many researchers owing to its extensive industrial applications. The search for novel mannanases with...
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Cross-linked β-Mannanase Aggregates: Preparation, Characterization, and Application for Producing Partially Hydrolyzed Guar Gum
β-Mannanase capable of hydrolyzing β-1,4-linkages in guar gum was immobilized as cross-linked enzyme aggregates (M-CLEAs). The aggregation and...
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Resha** the binding channel of a novel GH113 family β-mannanase from Paenibacillus cineris (PcMan113) for enhanced activity
Endo-β-mannanases are important enzymes for degrading lignocellulosic biomass to generate mannan, which has significant health effects as a prebiotic...
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The Increase of Incomplete Degradation Products of Galactomannan Production by Synergetic Hydrolysis of β-Mannanase and α-Galactosidase
An integrated process to increase the yield of incomplete degradation products of galactomannan (GalM) especially for galactomanno-oligosaccharides...
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Production and characterization of thermostable acidophilic β-mannanase from Aureobasidium pullulans NRRL 58524 and its potential in mannooligosaccharide production from spent coffee ground galactomannan
The maximum yield of the crude mannanase from Aureobasidium pullulans NRRL 58524 was 8.42 ± 0.18 U mL −1 when cultured for 72 h in the optimized...
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High-level expression of a β-mannanase (manB) in Pichia pastoris GS115 for mannose production with Penicillium brevicompactum fermentation pretreatment of soybean meal
AbstractAn endo-1,4-β-mannanase gene (manB) from a Bacillus pumilus Nsic-2 grown in a stinky tofu emulsion was cloned and expressed in Pichia pastoris ...
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Expression, homology modeling and enzymatic characterization of a new β-mannanase belonging to glycoside hydrolase family 1 from Enterobacter aerogenes B19
Backgroundβ-mannanase can hydrolyze β-1,4 glycosidic bond of mannan by the manner of endoglycosidase to generate mannan-oligosaccharides. Currently,...
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Identification and Biochemical Characterization of Major β-Mannanase in Talaromyces cellulolyticus Mannanolytic System
Talaromyces cellulolyticus is a promising fungus for providing a cellulase preparation suitable for the hydrolysis of lignocellulosic material,...
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Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei
The fungus Trichoderma reesei is a powerful host for secreted production of proteins. The promoter of cdna1 gene, which encodes a small basic protein...
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Using One-pot Fermentation Technology to Prepare Enzyme Cocktail to Sustainably Produce Low Molecular Weight Galactomannans from Sesbania cannabina Seeds
Enzymatic hydrolysis using β-mannanase and α-galactosidase is necessary to produce low molecular weight galactomannan (LMW-GM) from galactomannans...
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Effects of Konjac Glucomannan with Different Molecular Weights on Functional and Structural Properties of κ-carrageenan Composite Gel
In this study, the properties of konjac glucomannan with different molecular weights and their effects on the functional and structural properties of...
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Coordinately express hemicellulolytic enzymes in Kluyveromyces marxianus to improve the saccharification and ethanol production from corncobs
BackgroundHemicellulose acts as one factor contributing to the recalcitrance of lignocellulose that prevents cellulases to degrade the cellulose...
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The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus
The filamentous fungus Talaromyces cellulolyticus is a well-characterized cellulolytic and hemicellulolytic enzyme producer. In this study, the...
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Copra meal hydrolysis by the recombinant β-mannanase KMAN-3 and MAN 6.7 expressed in Escherichia coli
Hydrolysis products of defatted copra meal (DCM) hydrolysis were investigated with either recombinant β-mannanases from Klebsiella oxytoca KUB-CW2-3...
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Penicillium polonicum a new isolate obtained from Cerrado soil as a source of carbohydrate-active enzymes produced in response to sugarcane bagasse
Penicillium species have been studied as producers of plant cell wall degrading enzymes to deconstruct agricultural residues and to be applied in...
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A novel pressed coal from citrus and cooking oil wastes using fungi
Nowadays renewable energy with low prices is a global target that has taken the attention to compare alternatives energy sources with fossil fuels....
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Improved enzyme production on corncob hydrolysate by a xylose-evolved Pichia pastoris cell factory
The global shift from the usage of crude oil in bio-production is receiving much attention owing to environmental concern associated with fossil...
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Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum
BackgroundLactic acid bacteria (LAB) are important microorganisms in the food and beverage industry. Due to their food-grade status and probiotic...
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Strategies for Quality Control of Polysaccharides in Chinese Medicines
Polysaccharides with multiple biological activities are usually considered as one of the major bioactive compounds in Chinese medicines (CMs). At...
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Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity
BackgroundPreviously, we isolated a mutant of Parachlorella kessleri named strain PK4 that accumulated higher concentrations of lipids than the...
