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Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst
BackgroundTrigonopsis variabilis D -amino acid oxidase ( Tv DAO) is a well characterized enzyme used for cephalosporin C conversion on industrial...
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Maximizing filamentous phage yield during computer-controlled fermentation
Filamentous phage such as M13 and fd consist of a circular, single-stranded DNA molecule surrounded by several different coat proteins. These phages...
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Large Scale Production of Stem Cells and Their Derivatives
Stem cells have been envisioned to become an unlimited cell source for regenerative medicine. Notably, the interest in stem cells lies beyond direct... -
Fully automated single-use stirred-tank bioreactors for parallel microbial cultivations
Single-use stirred tank bioreactors on a 10-mL scale operated in a magnetic-inductive bioreaction block for 48 bioreactors were equipped with...
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Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production
BackgroundIn cell culture-based influenza vaccine production the monitoring of virus titres and cell physiology during infection is of great...
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Configuration of Bioreactors
For basic research on animal cells and in process development and troubleshooting, lab-scale bioreactors (0.2–20 L) are used extensively. In this... -
A new wireless system for decentralised measurement of physiological parameters from shake flasks
BackgroundShake flasks are widely used because of their low price and simple handling. Many researcher are, however, not aware of the physiological...
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Miniature bioreactors: current practices and future opportunities
This review focuses on the emerging field of miniature bioreactors (MBRs), and examines the way in which they are used to speed up many areas of...
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Methods and milliliter scale devices for high-throughput bioprocess design
Based on electromagnetic simulations as well as on computational fluid dynamics simulations gas-inducing impellers and their magnetic inductive drive...
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Enabling Technologies: Fermentation and Downstream Processing
Efficient parallel tools for bioprocess design, consequent application of the concepts for metabolic process analysis as well as innovative... -
High Throughput Transient Transfections of Hek293Ebna Cells — Devel Opment of an Integrated Expression Purification Process at 100 ml Scale
This work describes the set-up and optimisation of a simple, robust and cost effective process for the high throughput production of recombinant... -
Integrated L-phenylalanine separation in an E. coli fed-batch process: from laboratory to pilot scale
Pilot-scale reactive-extraction technology for fully integrated L-phenylalanine (L-Phe) separation in Escherichia coli fed-batch fermentations was...
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Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction
A fully integrated process for the microbial production and recovery of the aromatic amino acid L-phenylalanine is presented. Using a recombinant...