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Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures
BackgroundIndustrial processes for recombinant protein production challenge production hosts, such as the yeast Pichia pastoris , on multiple levels....
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Optimization of the biotechnological production of a novel class of anti-MRSA antibiotics from Chitinophaga sancti
BackgroundRecently, the discovery of the elansolids, a group of macrolides, was reported. The molecules show activity against methicillin-resistant St...
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Isolation of a genetically accessible thermophilic xylan degrading bacterium from compost
BackgroundDue to the finite nature of global oil resources we are now faced with the challenge of finding renewable resources to produce fuels and...
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Systems metabolic engineering of Corynebacterium glutamicum for the production of the carbon-5 platform chemicals 5-aminovalerate and glutarate
BackgroundThe steadily growing world population and our ever luxurious life style, along with the simultaneously decreasing fossil resources has...
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New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica
BackgroundThe oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At...
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A novel toolbox for E. coli lysis monitoring
The bacterium Escherichia coli is a well-studied recombinant host organism with a plethora of applications in biotechnology. Highly valuable...
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Metabolic engineering of Saccharomyces cerevisiae to produce a reduced viscosity oil from lignocellulose
BackgroundAcetyl-triacylglycerols (acetyl-TAGs) are unusual triacylglycerol (TAG) molecules that contain an sn -3 acetate group. Compared to typical...
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Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a model product
BackgroundFilamentous fungi are commonly used as production hosts for bulk enzymes in biotechnological applications. Their robust and quick growth...
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Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775
This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia...
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Systematic evaluation of characteristics of the membrane-based fed-batch shake flask
BackgroundThe initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation...
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Comparative evaluation of phototrophic microtiter plate cultivation against laboratory-scale photobioreactors
Extended cultivation times, rendering phototrophic bioprocess development time inefficient, resulted in the recent development of...
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Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
BackgroundPichia pastoris is a widely used eukaryotic expression host for recombinant protein production. Adaptive laboratory evolution (ALE) has...
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High-Throughput Process Development for Biopharmaceuticals
The ability to conduct multiple experiments in parallel significantly reduces the time that it takes to develop a manufacturing process for a...
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Identification of target genes to control acetate yield during aerobic fermentation with Saccharomyces cerevisiae
BackgroundAerobic fermentation of grape must, leading to respiro-fermentative metabolism of sugars, has been proposed as way of reducing alcohol...
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Catabolism of coniferyl aldehyde, ferulic acid and p-coumaric acid by Saccharomyces cerevisiae yields less toxic products
BackgroundLignocellulosic substrates and pul** process streams are of increasing relevance to biorefineries for second generation biofuels and...
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Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei
BackgroundThe filamentous fungus Trichoderma reesei has tremendous capability to secrete over 100 g/L of proteins and therefore it would make an...
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Phenolics Value Chain and l-Lactic Acid Bioproduction from Agricultural Biomass
To make the quantum leap from conceptualization of an idea to possible commercialization of a product, at first it would be desirable to have the...
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Industrial Biotechnology: Discovery to Delivery
Bioprocessing technologies have been evolving over the past several decades with fermentation products finding applications in almost every aspect of...
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Systems analysis of methylerythritol-phosphate pathway flux in E. coli: insights into the role of oxidative stress and the validity of lycopene as an isoprenoid reporter metabolite
BackgroundHigh-throughput screening methods assume that the output measured is representative of changes in metabolic flux toward the desired product...
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Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates
BackgroundMicrobial lipids (triacylglycerols, TAG) have received large attention for a sustainable production of oleochemicals and biofuels. Rhodospor...
