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Protein Separation and Characterization Procedures
This chapter covers the principles, procedures, and applications of a variety of techniques used to separate, isolate, and characterize proteins....
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Investigation of Bioremediation Potential of Laccase Enzyme Immobilized on Silver-Coated Magnetic Nanoparticles
In this study, silver-coated magnetic nanoparticles were synthesized, functionalized with MUA, and covalently immobilized laccase enzyme. FTIR, TEM,...
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Obtaining and evaluating of enzymatic extracts from hairless canary seed (CDC Maria) as gluten-free bread-improving agents
In this study, enzyme extracts were obtained from hairless canary seeds (CDC Maria) and used as gluten-free bread improvers. The enzyme extraction...
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New trends in enzyme-free electrochemical sensing of ROS/RNS. Application to live cell analysis
The ubiquity and importance of ROS and RNS in cellular signaling, disease development, and death give rise to an outstanding interest in their...
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Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification
Bacillus pumilus , a bacterial strain was isolated from agricultural soil and used for xylanase enzyme (Xy) production under the submerged...
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Enzyme@bismuth-ellagic acid: a versatile platform for enzyme immobilization with enhanced acid-base stability
In situ encapsulation is an effective way to synthesize enzyme@metal-organic framework biocatalysts; however, it is limited by the conditions of...
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Protein Engineering of Lactate Oxidase
AbstractLactate oxidase is a practically important enzyme widely used to detect L-lactate in medical diagnostics and in the food industry. This...
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Proposed molecular mechanism of non-competitive inhibition using molecular dynamics simulations between α-glucosidase enzyme and mangostin compound as antidiabetic
ContextFurther understanding of the molecular mechanisms is necessary since it is important for designing new drugs. This study aimed to understand...
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Cell-Free Production and Regeneration of Cofactors
Cofactors, such as adenosine triphosphate, nicotinamide adenine dinucleotide, and coenzyme A, are involved in nearly 50% of enzymatic reactions and...
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Practical and Rapid Membrane-Based Biosensor for Phenol Using Copper/Calcium-Enzyme Hybrid Nanoflowers
Phenol, a pollutant frequently found in chemical industries effluents, is highly toxic even in low concentrations. This study reports a green,...
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Structural Information and Drug–Enzyme Interaction of the Non-Nucleoside Reverse Transcriptase Inhibitors Based on Computational Chemistry Approaches
Non-nucleoside reverse transcriptase inhibitors, such as nevirapine, TIBO, HEPT, and efavirenz, are very specific to HIV-1 reverse transcriptase...
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Label-Free Sample Preparation for Single-Cell Proteomics
In recent years, single-cell proteomics (SCP) has become a valuable addition to other single-cell omics technologies for studying cellular...
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Enzyme Kinetics Features of the Representative Engineered Recombinants of Chondroitinase ABC I
Chondroitinase ABC I (cABC I) from Proteus vulgaris is an important enzyme in medicinal biotechnology due to its ability to help axon regeneration...
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Cell-Free Production Systems in Droplet Microfluidics
The use of cell-free production systems in droplet microfluidic devices has gained significant interest during the last decade. Encapsulating DNA...
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Breaking free from free radicals: harnessing the power of natural antioxidants for health and disease prevention
Massive production of the free radicals disrupts the system of antioxidant defense in the animal body thereby causing damage to cellular molecules...
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In Vitro Determination of Protein Nutritional Indexes
Most of the recent studies investigating the effects of sourdough on baked goods have highlighted its role in improving different nutritional...
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High-Throughput Protein Expression Screening of Cell-Surface Protein Ectodomains
Cell-surface receptors can be difficult to express and purify for structural and biochemical studies due to low expression levels, misfolding,...
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Enzyme Assay Methods to Validate DIGE Proteomics Data
Enzyme activity assay methods can be used to corroborate the results generated by difference gel electrophoresis (DIGE) proteomic experiments. Two...
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Protein Stability: Enhancement and Measurement
This chapter defines protein stability, emphasizes its importance, and surveys the field of protein stabilization, with summary reference to a...
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Optimisation process of walnut protein hydrolysed as an antioxidant candidate
Walnut protein was extracted from defatted walnut meal, the preparation process of walnut protein antioxidant peptides was determined by response...
