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Evaluation of the Recombinant Bacterial Chitinases as Anti-proliferative and Anti-migratory Agents for the Human Breast Cancer Cell Line, MCF-7
Chitinases, a glycosyl hydrolase family 18 members, have a wide distribution in both prokaryotes and eukaryotes, including humans. Regardless of the...
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Revolutionizing Synthetic Antibody Design: Harnessing Artificial Intelligence and Deep Sequencing Big Data for Unprecedented Advances
Synthetic antibodies (Abs) represent a category of engineered proteins meticulously crafted to replicate the functions of their natural counterparts....
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Assessing the role of redox partners in TthLPMO9G and its mutants: focus on H2O2 production and interaction with cellulose
BackgroundThe field of enzymology has been profoundly transformed by the discovery of lytic polysaccharide monooxygenases (LPMOs). LPMOs hold a...
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Structure–function characterization of two enzymes from novel subfamilies of manganese peroxidases secreted by the lignocellulose-degrading Agaricales fungi Agrocybe pediades and Cyathus striatus
BackgroundManganese peroxidases (MnPs) are, together with lignin peroxidases and versatile peroxidases, key elements of the enzymatic machineries...
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Design and evolution of chimeric streptavidin for protein-enabled dual gold catalysis
Artificial metalloenzymes result from anchoring an organometallic catalyst within an evolvable protein scaffold. Thanks to its dimer of dimers...
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Biotechnological potential and initial characterization of two novel sesquiterpene synthases from Basidiomycota Coniophora puteana for heterologous production of δ-cadinol
BackgroundTerpene synthases are versatile catalysts in all domains of life, catalyzing the formation of an enormous variety of different terpenoid...
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Marine diterpenoid targets STING palmitoylation in mammalian cells
Natural products are important sources of therapeutic agents and useful drug discovery tools. The fused macrocycles and multiple stereocenters of...
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Assessing and engineering the IscB–ωRNA system for programmed genome editing
OMEGA RNA (ωRNA)-guided endonuclease IscB, the evolutionary ancestor of Cas9, is an attractive system for in vivo genome editing because of its...
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Alcohol acyltransferases for the biosynthesis of esters
Esters are widely used in food, energy, spices, chemical industry, etc., becoming an indispensable part of life. However, their production heavily...
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Improved PCR by the Use of Disruptors, a New Class of Oligonucleotide Reagents
As a powerful tool, polymerase chain reaction (PCR) has been indispensable and widely used in a large array of applications. In practice, many...
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Structure of GPR101–Gs enables identification of ligands with rejuvenating potential
GPR101 is an orphan G protein-coupled receptor actively participating in energy homeostasis. Here we report the cryo-electron microscopy structure of...
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Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis
BackgroundLycopene is a terpenoid pigment that has diverse applications in the food and medicine industries. A prospective approach for lycopene...
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In vivo Characterization of the Inducible Promoter System of 3-hydroxypropionic Dehydrogenase in Pseudomonas denitrificans
Here, we characterized a gene expression system induced by 3-hydroxypropionic acid (3-HP) in Pseudomonas denitrificans . The system consists of a...
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Redesigning Robust Biocatalysts by Engineering Enzyme Microenvironment and Enzyme Immobilization
Enzymes have replaced traditional industrial catalysts as more efficient, eco-friendly, and sustainable alternatives that can be used in different...
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Understanding the Specific Implications of Amino Acids in the Antibody Development
As the demand for immunotherapy to treat and manage cancers, infectious diseases and other disorders grows, a comprehensive understanding of amino...
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A Synopsis of Proteins and Their Purification
The goal of protein purification is to separate a specific protein from all other biomolecules. Classical chromatographic procedures have been...
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CRISPR/Cas9-mediated editing of phytoene desaturase (PDS) gene in an important staple crop, potato
The gene editing using the CRISPR/Cas9 system has become an important biotechnological tool for studying gene function and improving crops. In this...
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Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR)
A large number of beneficial substitutions can be obtained from a successful directed enzyme evolution campaign and/or (semi)rational design. It is...
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Synthetic auxotrophs accelerate cell factory development through growth-coupled models
The engineering of microbial cell factories for the production of high-value chemicals from renewable resources presents several challenges,...
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Yeast Cell Factory for Production of Biomolecules
In the 1860s, Louis Pasteur first demonstrated the relationship between wine fermentation and yeast, which was later studied by different scientists...