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Genomic and transcriptomic analysis of Candida intermedia reveals the genetic determinants for its xylose-converting capacity
BackgroundAn economically viable production of biofuels and biochemicals from lignocellulose requires microorganisms that can readily convert both...
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Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin
BackgroundCis , cis -muconic acid (MA) is a dicarboxylic acid of recognized industrial value. It provides direct access to adipic acid and...
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Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae
IntroductionPhosphoketolases (Xfpk) are a non-native group of enzymes in yeast, which can be expressed in combination with other metabolic enzymes to...
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Metabolic engineering of Bacillus megaterium for heparosan biosynthesis using Pasteurella multocida heparosan synthase, PmHS2
BackgroundHeparosan is the unsulfated precursor of heparin and heparan sulfate and its synthesis is typically the first step in the production of...
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Multidimensional engineering of Saccharomyces cerevisiae for efficient synthesis of medium-chain fatty acids
Medium-chain fatty acids (MCFAs; C6–C12) are valuable molecules used for biofuel and oleochemical production; however, it is challenging to...
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Engineering E. coli for improved microaerobic pDNA production
Escherichia coli strains W3110 and BL21 were engineered for the production of plasmid DNA (pDNA) under aerobic and transitions to microaerobic...
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Discovery and implementation of a novel pathway for n-butanol production via 2-oxoglutarate
BackgroundOne of the European Union directives indicates that 10% of all fuels must be bio-synthesized by 2020. In this regard, biobutanol—natively...
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Changes in lipid metabolism convey acid tolerance in Saccharomyces cerevisiae
BackgroundThe yeast Saccharomyces cerevisiae plays an essential role in the fermentation of lignocellulosic hydrolysates. Weak organic acids in...
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Custom made inclusion bodies: impact of classical process parameters and physiological parameters on inclusion body quality attributes
BackgroundThe bacterium E. coli is a major host for recombinant protein production of non-glycosylated products. Depending on the expression...
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Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
BackgroundThe Gram-positive Streptomyces lividans TK24 is an attractive host for heterologous protein production because of its high capability to...
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E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)
BackgroundEscherichia coli is one of the most widely used hosts for recombinant protein production in academia and industry. Strain BL21(DE3) is...
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Identification of sesquiterpene synthases from the Basidiomycota Coniophora puteana for the efficient and highly selective β-copaene and cubebol production in E. coli
BackgroundTerpenes are an important and extremely versatile class of secondary metabolites that are commercially used in the pharmaceutical, food and...
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Awakening slee** beauty: production of propionic acid in Escherichia coli through the sbm operon requires the activity of a methylmalonyl-CoA epimerase
BackgroundPropionic acid is used primarily as a food preservative with smaller applications as a chemical building block for the production of many...
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Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis
BackgroundEfficient microbial production of chemicals is often hindered by the cytotoxicity of the products or by the pathogenicity of the host...
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Characterizing the effect of expression of an acetyl-CoA synthetase insensitive to acetylation on co-utilization of glucose and acetate in batch and continuous cultures of E. coli W
BackgroundDue to its high stress tolerance and low acetate secretion, Escherichia coli W is reported to be a good production host for several...
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Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses
BackgroundUnlike the well-studied backer yeast where catabolite repression represents a burden for mixed substrate fermentation, Yarrowia lipolytica ,...
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Mixing theory for culture and harvest in bioreactors of human mesenchymal stem cells on microcarriers
The use of human mesenchymal stem cells (hMSCs) in regenerative medicine is a potential major advance for the treatment of many medical conditions,...
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Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast
BackgroundAssembly of heterologous metabolic pathways is commonly required to generate microbial cell factories for industrial production of both...
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Large-scale production of a thermostable Rhodothermus marinus cellulase by heterologous secretion from Streptomyces lividans
BackgroundThe gene encoding a thermostable cellulase of family 12 was previously isolated from a Rhodothermus marinus through functional screening....
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3-Hydroxypropionaldehyde production from crude glycerol by Lactobacillus diolivorans with enhanced glycerol uptake
BackgroundIn their quest for sustainable development and effective management of greenhouse gas emissions, our societies pursue a shift away from...
