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Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae
BackgroundThe physiological characterization of microorganisms provides valuable information for bioprocess development. Chemostat cultivations are a...
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Palladium-Catalyzed Cross-Coupling Reactions in the Synthesis of Pharmaceuticals
The use of Pd-catalyzed cross-coupling methods for the construction of C-C, C-N, and C-O bonds have increased exponentially over the years. The... -
Analysis of volumetric mass transfer coefficient (kLa) in small- (250 mL) to large-scale (2500 L) orbitally shaken bioreactors
In this study, the combination of dimensional analysis (DA) and analysis of variance (ANOVA) was used to predict the volumetric mass transfer...
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High-resolution Grazing Incidence Small Angle X-ray Scattering: Investigation of Micrometer Sized Structured Polymer Films
While bulk samples are routinely probed by small angle X-ray scattering (SAXS) and ultra-small angle X-ray scattering (USAXS), the grazing... -
Small-Scale Technology for Benzene Synthesis from Coke-Oven Gas
AbstractA small-scale production technology for benzene, toluene, xylenes, thiophene, saturated hydrocarbons, polymers, thiourea, and ammonium...
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Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System
Purification of proteins for the biophysical analysis of protein interactions occurring in human cells can benefit from methods that facilitate the... -
Case Study: Description and Analysis for Corrosion Main Features
The proposed approach shown in Part I will be implemented in a real case study based on the results from two consecutive In-Line Inspections 2 years... -
Downstream Processes for Cultivated Meat
Cultivated meat production is being developed worldwide and is gaining traction. Whether the technology becomes a staple in the food industry,... -
A Machine Learning Approach for Predicting Essentiality of Metabolic Genes
The identification of essential genes is a key challenge in systems and synthetic biology, particularly for engineering metabolic pathways that... -
Scaling-Up of Cultivated Meat Production Process
Upscaling the production of cultured meat from laboratory flasks to an industrial scale is undeniably a great challenge, yet it is imperative for the... -
Scale-Up of Protein Purification: Downstream Processing Issues
Large-scale chromatography operations continue to occupy the central position in the overall strategy for downstream processing and purification of... -
Production and Purification of Antibodies in Chinese Hamster Ovary Cells
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of... -
Transient Co-expression of Membrane Protein Complexes in Mammalian Cells
Membrane proteins are essential components of biological membranes with key roles in cellular processes such as nutrient transport, cell... -
Industrial Microbiology Based Entrepreneurship Making Money from Microbes
This book is first part of the 3 volume set focusing on basic and advanced methods for using microbiology as an entrepreneurial venture. This book... -
The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production
Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production... -
Socio-economic Implications of Deploying Sustainable Phosphorus Management Systems
Phosphorus causes nutrient pollution in lentic water systems, which act as a reservoir of phosphorus since it is stored within the sediments of these... -
Numerical study of multicomponent mixed refrigerant composition regulation during natural gas liquefaction
One of the promising directions of energy efficiency enhancement and operating cost reduction when small-scale natural gas liquefaction plants where...
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Natural and low-cost sorbents as part of the solution for biogas upgrading: A review
The availability of pollutants in biogas especially carbon dioxide hinders its application in the enginery parts by minimizing its calorific...
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Current Understanding on the Unique Relaxation Dynamics of Sub-nanometer Materials and Their Structure-Property Relationships
Sub-nanometer materials(SNMs) define the emergent functional material system with their characteristic dimensions at sub-1 nm scale and they can be...
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Scale-up analysis of a twin-bed PSA pilot plant
Small-scale pressure swing adsorption (PSA) plants, also referred to as pilot plants, are commonly exploited for studying separation processes in...