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Specific capture and determination of glycoprotein using a hybrid epitopes and monomers-mediated molecular-imprinted polymer enzyme-free electrochemical biosensor
A novel molecular-imprinted polymer (MIP)–based enzyme-free biosensor was created for the selective detection of glycoprotein transferrin (Trf). For...
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Enzyme-Assisted Extraction
The extraction of compounds from plant and animal sources that have medicinal properties are known as bioactive compounds, which can either improve...
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Assessing and optimizing the bioactivities of diverse enzyme-derived protein hydrolysates from Porphyra yezoensis: unlocking the health potential
The interest in algae-derived bioactive compounds has grown due to their potential therapeutic efficacy against a range of diseases. These compounds,...
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Immobilized enzyme cascade for targeted glycosylation
Glycosylation is a critical post-translational protein modification that affects folding, half-life and functionality. Glycosylation is a...
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Computational Protein Binding
Why it is important to know this material? The computational approach to protein binding has become a crucial methodology in drug design and...
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Computational Methods to Target Protein-Protein Interactions
The chapter emphasizes the importance of understanding protein-protein interactions in cellular mechanisms and highlights the role of computational...
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Enzyme-Based Nanomedicine for Cancer Therapy
Tremendous advancements in the field of nanotechnology have led to the fabrication of novel nanomaterials that ensure cancer specific targeting and...
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Enzyme engineering for functional lipids synthesis: recent advance and perspective
Functional lipids, primarily derived through the modification of natural lipids by various processes, are widely acknowledged for their potential to...
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Stress Induced by Recombinant Protein Production in Escherichia coli
Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to...
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Loop dynamics and the evolution of enzyme activity
In the early 2000s, Tawfik presented his ‘New View’ on enzyme evolution, highlighting the role of conformational plasticity in expanding the...
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Simple Enzyme-Free Biosensor for Highly Sensitive and Selective Detection of miR-21 Based on Multiple Signal Amplification Strategy
Due to the fact that most microRNAs are small in size, low abundance in biological samples, homologous sequence among family members, and protein...
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Protein-Ligand Interactions and Binding
Why it is important to know this material? Protein binding is implied in all biological mechanisms, and the definition of quantitative models and...
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Alginate as Support Material in Enzyme Immobilization
Despite the extensive application of enzymes in various industries, their instability and non-reusability cause to their limitation. The...
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Label-Free Quantitative Proteomics of Oral Microbial Communities
The oral cavity is a habitat for different microorganisms, of which bacteria are best described. Studying different bacterial taxa and their proteins...
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Improved catalytic performance of carrier-free immobilized lipase by advanced cross-linked enzyme aggregates technology
The cross-linked enzyme aggregates (CLEAs) are one of the technologies that quickly immobilize the enzyme without a carrier. In this study, ionic...
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Groundnut Meal: Scientific Interventions for Achieving Superior Quality of Protein
Groundnut is protein rich oilseed crop widely grown in India for its pods and oil. Several by-products are obtained from crushed and harvested...
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Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pastoris strain expressing a levansucrase-endolevanase fusion enzyme
BackgroundAlthough Levan-type fructooligosaccharides (L-FOS) have been shown to exhibit prebiotic properties, no efficient methods for their...
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Enzyme Conjugation - A Promising Tool for Bio-catalytic and Biotransformation Applications – A Review
Enzymes have revolutionized conventional industrial catalysts as more efficient, eco-friendly, and sustainable substitutes that can be used in...
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Protein Digestion for 2D-DIGE Analysis
In-gel digestion of protein spots derived from two-dimensional gels and their subsequent identification by mass spectrometry is involved in a...
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Cell-Free Systems for the Production of Glycoproteins
Cell-free protein synthesis (CFPS), whereby cell lysates are used to produce proteins from a genetic template, has matured as an attractive...