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Screening for optimal protease producing Bacillus licheniformis strains with polymer-based controlled-release fed-batch microtiter plates
BackgroundSubstrate-limited fed-batch conditions have the favorable effect of preventing overflow metabolism, catabolite repression, oxygen...
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Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor
In recent years, the use of hMSCs, which may be isolated from adipose tissue among others, for the treatment of diseases has increased significantly....
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Reduction of charge variants by CHO cell culture process optimization
Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents,...
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Metabolic control analysis enables rational improvement of E. coli l-tryptophan producers but methylglyoxal formation limits glycerol-based production
BackgroundAlthough efficient l -tryptophan production using engineered Escherichia coli is established from glucose, the use of alternative carbon...
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Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose
Background5-Ketofructose (5-KF) has recently been identified as a promising non-nutritive natural sweetener. Gluconobacter oxydans strains have been...
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Adaptation of central metabolite pools to variations in growth rate and cultivation conditions in Saccharomyces cerevisiae
BackgroundSaccharomyces cerevisiae is a well-known popular model system for basic biological studies and serves as a host organism for the...
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Identification of a Yarrowia lipolytica acetamidase and its use as a yeast genetic marker
BackgroundYarrowia lipolytica is an oleaginous yeast that can be genetically engineered to produce lipid and non-lipid biochemicals from a variety...
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Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production
BackgroundEngineering efforts targeted at increasing ethanol by modifying the central fermentative metabolism of Clostridium thermocellum have been...
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Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential
PurposeBrewers’ spent grain (BSG) represents the largest by-product of the brewing industry. Its utilisation as an animal feed has become less...
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Co-cultures with integrated in situ product removal for lactate-based propionic acid production
Propionic acid (PA) is a valuable organic acid for the food and feed industry, but no bioproduction at industrial scale exists so far. As product...
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Growth-dependent recombinant product formation kinetics can be reproduced through engineering of glucose transport and is prone to phenotypic heterogeneity
BackgroundEscherichia coli W3110 and a group of six isogenic derivatives, each displaying distinct specific rates of glucose consumption were...
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Studies on efficient production of a novel l-asparaginase by a newly isolated Pseudomonas resinovorans IGS-131 and its heterologous expression in Escherichia coli
In the current study, the production of novel glutaminase free l -asparaginase from a new microbial source ( Pseudomonas resinovorans IGS-131) is...
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Metabolic control analysis of L-tryptophan producing Escherichia coli applying targeted perturbation with shikimate
L-tryptophan production from glycerol with Escherichia coli was analysed by perturbation studies and metabolic control analysis. The insertion of a...
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Production of 10-methyl branched fatty acids in yeast
BackgroundDespite the environmental value of biobased lubricants, they account for less than 2% of global lubricant use due to poor thermo-oxidative...
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The impact of technical failures during cultivation of an inclusion body process
In biotechnological processes, technical failures in the upstream process often lead to batch loss. It is of great interest to investigate the...
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Microbial 2-butanol production with Lactobacillus diolivorans
BackgroundBiobutanol has great potential as biofuel of the future. However, only a few organisms have the natural ability to produce butanol. Amongst...
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Engineering Escherichia coli FAB system using synthetic plant genes for the production of long chain fatty acids
BackgroundSustainable production of microbial fatty acids derivatives has the potential to replace petroleum based equivalents in the chemical,...
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Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
BackgroundThe yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we...
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Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production
The global demand for complex biopharmaceuticals like recombinant proteins, vaccines, or viral vectors is steadily rising. To further improve process...
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Efficient Development of a Mixed Feed Process for Pichia pastoris
Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. A prominent...