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Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototy**
Biological systems provide a sustainable and complimentary approach to synthesizing useful chemical products. Metabolic engineers seeking to...
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Effect of enzyme hydrolysis on the physicochemical, functional, and nutritional properties of pea and faba bean protein isolates
This research examined the physicochemical, functional, and nutritional aspects of pea (PPI) and faba bean protein isolates (FBPI) modified using...
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eCell Technology for Cell-Free Protein Synthesis, Biosensing, and Remediation
The eCell technology is a recently introduced, specialized protein production platform with uses in a multitude of biotechnological applications....
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Sensitive and Enzyme-Free Pathogenic Bacteria Detection Through Self-Circulation of Molecular Beacon
This research exhibits the design of a feasible, enzyme-free and sensitive fluorescent sensing assay for the detection of Staphylococcus aureus ( S....
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Chimeric Protein Switch Biosensors
Rapid detection of protein and small-molecule analytes is a valuable technique across multiple disciplines, but most in vitro testing of biological...
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The Use of Cell-free Protein Synthesis to Push the Boundaries of Synthetic Biology
Cell-free protein synthesis is emerging as a powerful tool to accelerate the progress of synthetic biology. Notably, cell-free systems that harness...
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A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression
With the rapid development of cell-free biotechnology, more and more cell-free protein synthesis (CFPS) systems have been established and optimized...
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Supramolecular protein assembly in cell-free protein synthesis system
Protein-based biomaterials have the characteristics of stability and biocompatibility. Based on these advantages, various bionic materials have been...
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Cell-Free Protein Synthesis Using Pichia pastoris
Pichia pastoris (syn. Komagataella phaffii) is an industrially relevant recombinant protein platform that has been used to produce over 5000 proteins...
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Accessible light-controlled knockdown of cell-free protein synthesis using phosphorothioate-caged antisense oligonucleotides
Controlling cell-free expression of a gene to protein with non-invasive stimuli is vital to the future application of DNA nanodevices and synthetic...
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The maximal and current accuracy of rigorous protein-ligand binding free energy calculations
Computational techniques can speed up the identification of hits and accelerate the development of candidate molecules for drug discovery. Among...
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Evaluating the properties of the hydrolyzed clover sprout protein obtained by enzymatic hydrolysis and its effect in free and micro-encapsulated form on the properties of probiotic yogurt
The purpose of this study was to investigate the effect of clover sprout protein hydrolyzed (CPH) in free and micro-encapsulated form on the quality...
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Label-free quantification of host cell protein impurity in recombinant hemoglobin materials
Quantitative analysis relies on pure-substance primary calibrators with known mass fractions of impurity. Here, label-free quantification (LFQ) is...
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Modeling Enzyme-Substrate Interaction with Localization-Delocalization Matrices
Enzymes are life’s catalysts par excellence. They are incredibly efficient and are mainly composed of protein—save prosthetic groups and/or nucleic...
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Redesigning Robust Biocatalysts by Engineering Enzyme Microenvironment and Enzyme Immobilization
Enzymes have replaced traditional industrial catalysts as more efficient, eco-friendly, and sustainable alternatives that can be used in different...
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Inexpensive and colorimetric RNA detection at ambient temperature with a cell-free protein synthesis platform
While many proposals of paper-based diagnostics utilize cell-free gene expression systems, these assays oftentimes suffer from the need for...
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Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
BackgroundThe filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading...
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Antioxidant and angiotensin-I-converting enzyme (ACE-I) inhibitory activities of protein hydrolysates derived from water buffalo (Bubalus bubalis) liver
In the current study, we attempted to use ginger as a novel and natural source of protease in comparison with other commercially available enzymes to...
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Protein Structure Prediction and Analysis
Why it is important to know this material? Protein structure prediction is one of the hot topics of the modern computational biology. The...
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Protein Analysis
Protein content analysis of foods and ingredients is important for a variety of reasons. This chapter covers the instrumentation, principles,...