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Production of monoclonal antibody against tylosin and tilmicosin with homogeneous cross-reactivity and its application in lateral flow immunoassay
To avoid false negative results due to the low cross-reactivity rate (CR) in rapid immunoassay, a group-specific antibody with homogeneous CR toward...
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One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher
One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the...
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A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the...
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Self-propelled Janus nanomotor as active probe for detection of pepsinogen by lateral flow immunoassay
The feasibility of using nanomotors as active probes for lateral flow immunoassay (LFIA) is demonstrated. We synthesized Au@mSiO 2 @Pt Janus nanomotor,...
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A Fluorescence Polarization Immunoassay Procedure for Determining Dibutyl Phthalate in Water
AbstractWe developed a fluorescence polarization immunoassay (FPIA) procedure for determining the dibutyl phthalate (DBP) plasticizer, a toxic...
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Upconversion nanoparticles-based background-free selective fluorescence sensor developed for immunoassay of fipronil pesticide
In this study, high luminous efficiency, sensitive and specific fluorescent immunosensor was proposed for the detection of fipronil in spinach...
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Inflammasome Activation in Human Macrophages: IL-1β Cleavage Detection by Fully Automated Capillary-Based Immunoassay
Interleukin (IL)-1β is a key mediator of inflammation and activates via pattern recognition receptors (PRR) of the inflammasome family by proteolytic...
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Fluorescent enzyme-linked immunosorbent assay based on alkaline phosphatase-responsive coordination polymer composite
The fabrication of alkaline phosphatase (ALP)-responsive coordination polymer (CP) composite is demonstrated for establishing a fluorescent...
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Nickel Nanocluster as a Fluorescent Probe for the Non-enzymatic Detection of Cardiac Troponin I
Troponin I, as a cardiac-specific biomarker, is a very valuable tool in the identification of acute coronary syndromes. In this work, an economical...
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Rapid and sensitive detection of E. coli O157:H7 by lateral flow immunoassay and silver enhancement
AbstractThe aim of this study was to develop a sensitive lateral flow immunoassay (LFIA) for the rapid detection of Escherichia coli ( E. coli )...
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Microfluidic synthesis of Janus-structured QD-encoded magnetic microbeads for multiplex immunoassay
Uniform and monodisperse quantum dot (QD)–encoded magnetic microbeads with Janus structure were produced in a microfluidic device via...
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Bioluminescent Bridging Immunoassay for Anti-Drug Antibody (ADA) Detection
Any immune reaction to therapeutic antibodies will impact the drug efficacy and can have serious consequences for patient safety. Therefore,...
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A competitive immunoassay based on engineered magnetic/fluorescent nanoparticles and biolayer interferometry-based assay for T-2 toxin determination
For the first time a competitive immunoassay was developed by employing T-2 antibody-functionalized magnetite nanoparticles and T-2 toxin-conjugated...
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Development of cellulosic material-based microchannel device capable of fluorescence immunoassay of microsamples
Microfluidic immunoassay devices are a promising technology that can quickly detect biomarkers with high sensitivity. Recently, many studies...
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Smartphone-based paper microfluidic competitive immunoassay for the detection of α-amanitin from mushrooms
α-Amanitin is often considered the most poisonous mushroom toxin produced by various mushroom species, which are hard to identify from edible,...
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Fluorescent Biosensing and Chemosensing Strategies for Food Quality Assessment
In develo** countries like India the population scale has been gradually increasing year by year and hence the need to supply healthy or hygienic...
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Fluorescent Lateral Flow Assay with Carbon Nanodot Conjugates for Carcinoembryonic Antigen
A lateral flow assay (LFA) sensor on a half-strip platform labeled with blue carbon nanodot-polystyrene (PS-CND) nanoconjugates was developed for the...
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A label-free ratiometric fluorescent aptasensor based on a peroxidase-mimetic multifunctional ZrFe-MOF for the determination of tetrodotoxin
A Fe/Zr bimetal-organic framework (ZrFe-MOF) is utilized to establish a ratiometric fluorescent aptasensor for the determination of tetrodotoxin...
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A simple and rapid homogeneous fluorescence polarization immunoassay for rapid identification of gutter cooking oil by detecting capsaicinoids
In order to address the widespread concerns with food safety such as adulteration and forgery in the edible oil field, this study developed a...
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Quantitative and simultaneous detection of two inflammation biomarkers via a fluorescent lateral flow immunoassay using dual-color SiO2@QD nanotags
An on-site detection strategy is reported based on dual-color SiO 2 @quantum dot (QD)–integrated lateral flow immunoassay (LFA) strip to realize the...
