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Clarification of Interactions among Microorganisms and Development of Co-culture System for Production of Useful Substances
Co-culture systems containing two microorganisms for the production of useful substances are described. We developed a novel co-culture system...
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Bacterial Capsular Polysaccharide and Sugar Transferases
Capsular polysaccharides (CPs) of several pathogenic bacteria are thought to be good materials for the development of new therapeutic reagents. These...
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Development of Culture Techniques of Keratinocytes for Skin Graft Production
The in vitro cultures of human tissues have attracted a great deal of medical attention as a promising technique for repairing defective tissues in...
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Development of Transition Metal-Mediated Cyclopropanation Reactions
A brief discussion of the methods for cyclopropane formation emphasizing organometallic reagents and catalysts is followed by several examples of the...
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Fluorescence Correlation Spectroscopy with Autofluorescent Proteins
Fluorescence correlation spectroscopy (FCS) is a versatile technique operating at the single-molecule level, that successfully meets many challenges...
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Inclusion Bodies: Formation and Utilisation
The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level production in bacterial hosts and simple optimisation...
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The Application of Multi-Parameter Flow Cytometry to Monitor Individual Microbial Cell Physiological State
The development of multi-parameter flow cytometric techniques in our laboratories has led to a functional classification of the physiological state...
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Roles of Heat-Shock Chaperones in the Production of Recombinant Proteins in Escherichia coli
Escherichia coli is a versatile organism for the production of recombinant proteins. Often, however, the recombinant protein does not reach its...
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Bacterial Sterilization and Intracellular Protein Release by a Pulsed Electric Field
Several biotechnological applications of high-voltage pulsed electric field (PEF) are introduced. Electrical breakdown or disruption of a biological...
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Functional Magnetic Particles for Medical Application
Magnetic particles for medical applications have been developed by many researchers. Since magnetic particles have unique magnetic features not...
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Metallocene-Catalyzed Selective Reactions
Transition metal cyclopentadienyl complexes constitute a large family of compounds that can be used as catalyst for a number of organic...
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Regulation of Cephalosporin Biosynthesis
The filamentous fungus Acremonium chrysogenum is the natural producer of the β-lactam antibiotic cephalosporin C and is as such used worldwide in...
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Facts and Theories of Induced Organ Regeneration
Induced organ regeneration is de novo synthesis of a physiological, or nearly physiological, organ at the same anatomical site as the organ that is...
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Thermal and mechanical characterization of ABS/15%PMMA co-extruded bilayer sheet
To study the service performance of polymer bilayer sheets, this experimental study focuses on the thermal and mechanical characterization of sheets...
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Investigation of medicinal plant processing waste for electricity generation through biomethanation
One of the waste-to-energy conversion technologies is the use of waste from the processing of medicinal plants to create biogas, which may then be...
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Production of activated carbons from Quercus cerris acorn shell under various experiment conditions, and their characterizations
Due to the high porosities, large surface areas, insolubilities in solutions, and unique structural and morphological structures, porous materials...
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Adeno-associated Virus as a Gene Therapy Vector: Vector Development, Production and Clinical Applications
Adeno-associated virus (AAV) has emerged as an attractive vector for gene therapy. AAV vectors have successfully been utilized to promote...
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Lentiviral Vectors
We review the use of lentiviral vectors in current human gene therapy applications that involve genetic modification of nondividing tissues with...