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Effects of site-directed mutagenesis of GLP-1 and glucagon receptors on signal transduction activated by dual and triple agonists
The paradigm of one drug against multiple targets, known as unimolecular polypharmacology, offers the potential to improve efficacy while overcoming...
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APOBEC3B regulates R-loops and promotes transcription-associated mutagenesis in cancer
The single-stranded DNA cytosine-to-uracil deaminase APOBEC3B is an antiviral protein implicated in cancer. However, its substrates in cells are not...
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N-Glycan Insertion for Probing Protein–Protein Interactions and Epitope Map**
Introducing an N-linked glycosylation motif into recombinant proteins at specific sites is a useful tool in probing protein–protein interactions and...
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APOBEC mutagenesis is a common process in normal human small intestine
APOBEC mutational signatures SBS2 and SBS13 are common in many human cancer types. However, there is an incomplete understanding of its stimulus,...
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DNA polymerase κ suppresses inflammation and inflammation-induced mutagenesis and carcinogenic potential in the colon of mice
BackgroundChronic inflammation induces DNA damage and promotes cell proliferation, thereby increasing the risk of cancer. DNA polymerase κ (Pol κ),...
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Activation of anthrachamycin biosynthesis in Streptomyces chattanoogensis L10 by site-directed mutagenesis of rpoB
Genome sequencing projects revealed massive cryptic gene clusters encoding the undiscovered secondary metabolites in Streptomyces. To investigate the...
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Deglycosylation of eukaryotic-expressed flagellin restores adjuvanticity
Flagellin, the TLR5 agonist, shows potent adjuvant activities in diverse vaccines and immunotherapies. Vibrio vulnificus flagellin B expressed in...
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Identification of target genes regulated by encystation-induced transcription factor Myb2 using knockout mutagenesis in Giardia lamblia
BackgroundEncystation is one of the two processes comprising the life cycle of Giardia lamblia , a protozoan pathogen with tetraploid genome. Giardia...
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Structural basis of tolvaptan binding to the vasopressin V2 receptor
The vasopressin V 2 receptor (V 2 R) is a validated therapeutic target for autosomal dominant polycystic kidney disease (ADPKD), with tolvaptan being...
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Biotechnology in Medicine: Fundamentals
Biotechnology is a diverse field with important implications in everyday life. It finds its use in research, medicine, agriculture, and chemical...
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Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis
To fully understand the function of cytomegalovirus (CMV) genes, it is imperative that they are studied in the context of infection. Therefore, the...
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Site-Directed Mutagenesis of Tryptophan Residues in the Structure of the Catalytic Module of Tyrosyl-tRNA Synthetase from Bos taurus
AbstractSite-directed mutagenesis of the N-terminal catalytic module of Bos taurus tyrosyl-tRNA synthetase (mini- Bt TyrRS) with the substitution of...
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Differential effects of mutations of POPDC proteins on heteromeric interaction and membrane trafficking
The Popeye domain containing (POPDC) genes encode sarcolemma-localized cAMP effector proteins. Mutations in blood vessel epicardial substance ( BVES )...
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Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus
Owing to the immunogenicity of adeno-associated viruses (AAVs), gene therapies using AAVs face considerable obstacles. Here, by leveraging ex vivo...
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AIM2 promotes irradiation resistance, migration ability and PD-L1 expression through STAT1/NF-κB activation in oral squamous cell carcinoma
BackgroundRadioresistance and lymph node metastasis are common phenotypes of refractory oral squamous cell carcinoma (OSCC). As a result,...
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Distal mutation V486M disrupts the catalytic activity of DPP4 by affecting the flap of the propeller domain
Dipeptidyl peptidase-4 (DPP4) plays a crucial role in regulating the bioactivity of glucagon-like peptide-1 (GLP-1) that enhances insulin secretion...
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Triclosan is a KCNQ3 potassium channel activator
KCNQ channels participate in the physiology of several cell types. In neurons of the central nervous system, the primary subunits are KCNQ2, 3, and...
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NLRP3 Phospho-residue Map** by Phospho Dot Blots
When characterizing posttranslational modifications like phosphorylation, using efficient screening methods to map the phospho sites is essential,...
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Identification of a novel interaction site between the large hepatitis delta antigen and clathrin that regulates the assembly of genotype III hepatitis delta virus
BackgroundHepatitis delta virus (HDV), a satellite virus of hepatitis B virus (HBV), is a small, defective RNA virus strongly associated with the...
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RedOx regulation of LRRK2 kinase activity by active site cysteines
Mutations of the human leucine-rich repeat kinase 2 (LRRK2) have been associated with both, idiopathic and familial Parkinson’s disease (PD). Most of...
