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A Restriction Endonuclease-Based Assay to Distinguish NANOGP8 Retrogene from Parental NANOG
NANOG is an embryonic transcription factor, which gets reexpressed in cancer stem or tumor initiating cells. NANOGP8, a retrogene belonging to the...
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Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns
BackgroundMobile group I introns encode homing endonucleases that confer intron mobility initiated by a double-strand break in the intron-lacking...
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MamA essentiality in Mycobacterium smegmatis is explained by the presence of an apparent cognate restriction endonuclease
ObjectiveRestriction-Modification (R-M) systems are ubiquitous in bacteria and were considered for years as rudimentary immune systems that protect...
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Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application for Glycobiology Research
The endonuclease activity of influenza A virus RNA polymerase allows the digestion of host mRNA. The PA endonuclease domain could be a target of...
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Domain definition and preliminary functional exploration of the endonuclease NOBP-1 in Strongyloides stercoralis
BackgroundRibosome biogenesis is the process of assembling ribosome complexes that regulate cell proliferation and differentiation with potential...
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Programmable Biosensors Based on RNA-Guided CRISPR/Cas Endonuclease
Highly infectious illnesses caused by pathogens constitute severe threats to public health and lead to global economic loss. The use of robust and...
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Construction of Geminivirus Infectious Clones for Agroinoculation into Plants
The production of geminiviral infectious clones provides a standardized inoculum for use in several host–virus studies. Geminiviruses present either...
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Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis
By using an inducible site-specific double-strand break (DSB) in budding yeast, it is possible to monitor—in real time—the repair of the break by...
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Determination of G/C Polymorphism at chr20:37352001 Position in Human Blood DNA Preparations by Methods of GlaI- and Bst2UI-PCR Analyses
AbstractSingle nucleotide polymorphism (SNP) is a change of one nucleotide by another. This change often leads to the emergence (or disappearance) of...
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Short-term calorie restriction enhances DNA repair by non-homologous end joining in mice
Calorie restriction (CR) improves health, reduces cancer incidence and extends lifespan in multiple organisms including mice. CR was shown to enhance...
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Inhibitors of bacterial immune systems: discovery, mechanisms and applications
To contend with the diversity and ubiquity of bacteriophages and other mobile genetic elements, bacteria have developed an arsenal of immune defence...
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CRISPR and CAS Editing Tools Employment in the Control of AMR Pathogens
Antibiotic-resistant bacteria have significantly emerged as a result of the widespread use of antibiotics in human and veterinary treatment....
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CRISPR and CAS Editing Tools Employent in the Control of AMR Pathogens
Antibiotic-resistant bacteria have significantly emerged as a result of the widespread use of antibiotics in human and veterinary treatment....
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Two novel phages, Klebsiella phage GADU21 and Escherichia phage GADU22, from the urine samples of patients with urinary tract infection
Phages are found in a wide variety of places where bacteria exist including body fluids. The aim of the present study was to isolate phages from the...
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Analysis of genetic diversity by the SLAF-seq among the farmed Onychostoma macrolepis populations
ObjectiveThe objective of this study was to examine the genetic diversity within and between farmed populations of Onychostoma macrolepis , and to...
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Characterization of the endonuclease activity of the replication-associated protein of beak and feather disease virus
Beak and feather disease virus (BFDV) belongs to the family Circoviridae . A rolling-circle replication strategy based on a replication-associated...
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Analysis of CHD Gene Polymorphism as a Model Object for Molecular Sexing of Eurasian Eagle-Owl (Bubo bubo)
AbstractCHD gene polymorphism as a model object for molecular sex determination in Bubo bubo (Eurasian eagle-owl) was studied. Classical PCR methods...
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An Introduction to Genome Editing Techniques
Understanding the genome and its function is crucial to understanding its role in health and disease. Genome editing—from inhibiting a gene to...
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Construction of Infectious Clones for Human Enteroviruses
The infectious clone has been constructed for years via various mechanisms using reverse genetics of viral RNA into cDNA. The mechanism of...
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Isolation and characterization of a novel phage belonging to a new genus against Vibrio parahaemolyticus
BackgroundVibrio parahaemolyticus is a major foodborne pathogen that contaminates aquatic products and causes great economic losses to aquaculture....
