Search
Search Results
-
A Guide to Using FASTPCR Software for PCR, In Silico PCR, and Oligonucleotide Analysis
The FastPCR software is an integrated tool environment for PCR primer and probe design and for prediction of oligonucleotide properties. The software...
-
RB1 screening of retinoblastoma patients in Sri Lanka using targeted next generation sequencing (NGS) and gene ratio analysis copy enumeration PCR (GRACE-PCR)
BackgroundRetinoblastoma (RB) a tumour affecting those under 5 years, has a prevalence of 1 in 20,000, with around twenty new diagnoses per year in...
-
Genomic Copy Number Analysis Using Droplet Digital PCR: A Simple Method with EvaGreen Single-Color Fluorescent Design
Droplet digital PCR (ddPCR) is an emerging method for the absolute quantification of PCR products, and it can detect DNA copy numbers accurately. It...
-
Microfluidic PCR and network analysis reveals complex tick-borne pathogen interactions in the tropics
BackgroundIxodid ticks, particularly Rhipicephalus sanguineus s.l., are important vectors of various disease-causing agents in dogs and humans in...
-
Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR
Analysis of immunoglobulin and T-cell receptor gene rearrangements by real-time quantitative polymerase chain reaction (RQ-PCR) is the gold standard...
-
Human papillomavirus spectrum of HPV-infected women in Nigeria: an analysis by next-generation sequencing and type-specific PCR
BackgroundHuman papillomavirus (HPV) infection and cervical cancer are leading health problems and causes of death in many parts of the world. There...
-
Genomic instability evaluation in different cell line by random amplified polymorphic DNA-PCR analysis
The cause of cancer is disorders and unstable genome. Some factors affect proper functioning of the DNA. These situations lead to cell death, cancer,...
-
Comparative performance of microscopy, nested PCR, and real-time PCR for screening avian haemosporidian parasites in Afrotropical starlings (family Sturnidae)
Prevalence studies of avian haemosporidian parasites frequently use microscopy and the nested polymerase chain reaction (PCR) protocols for detecting...
-
Multiplex PCR Design for Scalable Resequencing
While conventional PCR applications typically focus on a single PCR assay per reaction, multiplex PCR applications are a convenient and scalable...
-
Validation of Circular RNAs by PCR
High-throughput RNA-sequencing (RNA-seq) technologies combined with novel bioinformatic algorithms discovered a large class of covalently closed...
-
Diagnostic performance of mpox virus (MPXV) real-time PCR assays: multicenter assessment and extended sensitivity analysis
PurposeTo comprehensively investigate the diagnostic performance of routinely used assays in MPXV testing, the National Center of Clinical...
-
Detection of Rubella Virus by Tri-Primer RT-PCR Assay and Genoty** by Fragment RT-PCR
Polymerase chain reaction (PCR) is a widely used technique in the diagnosis of viral infections due to its low cost, high sensitivity, and...
-
Discrimination of SARS-CoV-2 omicron variant and its lineages by rapid detection of immune-escape mutations in spike protein RBD using asymmetric PCR-based melting curve analysis
BackgroundThe SARS-CoV-2 Omicron strain has multiple immune-escape mutations in the spike protein receptor-binding domain (RBD). Rapid detection of...
-
Self-swabbing versus assisted swabbing for viral detection by qRT-PCR: the experience from SARS-CoV-2 based on a meta-analysis of six prospectively designed evaluations conducted in a UK setting
PurposeIn April 2020, the UK Government implemented NHS Test and Trace to provide SARS-CoV-2 quantitative reverse transcription polymerase chain...
-
Analysis of var Gene Transcript Patterns by Quantitative Real-Time PCR
Quantitative real-time PCR (qPCR) is a simple and sensitive method for determining the amount of a specific target DNA sequence present in a sample....
-
Designing PCR Primers for the Amplification-Refractory Mutation System
The recent development in genetic research indicates that there exists intraspecific genetic variability in many organism groups. These variations,...
-
Genetic analysis challenges the presence of Ixodes inopinatus in Central Europe: development of a multiplex PCR to distinguish I. inopinatus from I. ricinus
BackgroundIxodes ricinus is an important vector of several pathogens, primarily in Europe. Recently, Ixodes inopinatus was described from Spain,...
-
PLASmid TAXonomic PCR (PlasTax-PCR), a Multiplex Relaxase MOB Ty** to Assort Plasmids into Taxonomic Units
Plasmids transmissible by conjugation are responsible for disseminating antibiotic-resistance genes, making plasmid detection relevant for pathogen...
-
Analysis of miRNA Expression Using Digital PCR and the QuantStudio™ 3D Digital PCR System
miRNAs are short, single-stranded RNA molecules that function as posttranscriptional regulators of gene expression. miRNAs represent ideal biomarkers...
-
Multiplex PCR for Diagnosis of Porcine Diseases
Molecular diagnostics have revolutionized the efficiency of diagnosis of infectious diseases. An accurate diagnosis of a disease would prevent...