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Western Blot Analysis in Ewing Sarcoma
Western blot is an experimental method used to analyze protein expression. In Ewing sarcoma, as in many other diseases, Western blot provides... -
Study of Circular RNA Expression by Nonradioactive Northern Blot Procedure
Circular RNAs (circRNAs) are covalently closed transcripts generated by back-splicing reaction. The lack of free ends endows these RNA molecules with... -
Empirical Validation of Overlap** Virus lncRNAs and Coding Transcripts by Northern Blot
Viruses, like their metazoan hosts, have evolved to utilize intricate transcriptional mechanisms to generate a vast array of both coding and... -
Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots
With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75% of the human genome is transcribed into RNAs.... -
From the ethnomedicinal plants in northern Indochina to the development of novel anti-cancer therapeutic agents
Northern Indochina is a region with abundant and diverse natural resources, including ethnomedicinal plants that have been traditionally used to...
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Molecular Analysis of Copy-Back Defective Interfering RNAs of Morbilliviruses
Copy-back defective interfering RNAs are major contaminants of viral stock preparations of morbilliviruses and other negative strand RNA viruses.... -
Genetic characteristics of a novel HIV-1 recombinant lineage (CRF103_01B) and its prevalence in northern China
During the routine surveillance of HIV-1 pretreatment drug resistance in Bei**g, five men who have sex with men (MSM) and a woman were observed to...
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The first genetically confirmed cohort of Facioscapulohumeral Muscular Dystrophy from Northern India
Facioscapulohumeral muscular dystrophy (FSHD) is the third most common form of hereditary myopathy. Sixty per cent of the world’s population lives in...
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Applications of Biotechnology in Pharmaceutical Product Analysis
The pharmaceutical sector is critical to supplying safe and effective pharmaceuticals to people all around the world. Pharmaceutical product... -
Presence of porcine cytomegalovirus, a porcine roseolovirus, in wild boars in Italy and Germany
Porcine cytomegalovirus (PCMV), a porcine roseolovirus (PRV) that is closely related to human herpesviruses 6 and 7, is commonly found in commercial...
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SDS-PAGE and Western Blotting: Basic Principles and Protocol
Western blotting is an important analytical technique used in cell and molecular biology for last four decades. It involves separation of proteins in... -
Intestinal recruitment of CCR6-expressing Th17 cells by suppressing miR-681 alleviates endotoxemia-induced intestinal injury and reduces mortality
IntroductionSepsis or endotoxemia can induce intestinal dysfunction in the epithelial and immune barrier. Th17 cells, a distinct subset of CD4 + ...
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ZNF148 inhibits HBV replication by downregulating RXRα transcription
BackgroundProgressive hepatitis B virus (HBV) infection can result in cirrhosis, hepatocellular cancer, and chronic hepatitis. While antiviral drugs...
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Protocols for Northern Analysis of Exosome Substrates and Other Noncoding RNAs
Over the past decade a plethora of noncoding RNAs (ncRNAs) have been identified, initiating an explosion in RNA research. Although RNA sequencing... -
ARHGAP17 Inhibits Hepatocellular Carcinoma Progression by Inactivation of Wnt/β-Catenin Signaling Pathway
As a member of Rho GAPs family, Rho GTPase-Activating Protein 17 (ARHGAP17) regulates cytoskeletal recombination, cell polarity, cell proliferation...
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Analysis of Mitochondrial Retrograde Signaling in Yeast Model Systems
Mitochondrial retrograde signaling is a mitochondria-to-nucleus communication pathway, conserved from yeast to humans, by which dysfunctional... -
Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediates
Retrozymes are a novel family of non-autonomous retrotransposable elements that contain hammerhead ribozyme motifs. These retroelements are found... -
Genetic meta-analysis of levodopa induced dyskinesia in Parkinson’s disease
The genetic basis of levodopa-induced-dyskinesia (LiD) is poorly understood, and there have been few well-powered genome-wide studies. We performed a...
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Identification and functional analysis of rare HECTD1 missense variants in human neural tube defects
Neural tube defects (NTDs) are severe malformations of the central nervous system that arise from failure of neural tube closure. HECTD1 is an E3...
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Expanding the clinical spectrum of biglycan-related Meester-Loeys syndrome
Pathogenic loss-of-function variants in BGN , an X-linked gene encoding biglycan, are associated with Meester-Loeys syndrome (MRLS), a thoracic aortic...