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Benchmark Glycan Profile of Therapeutic Monoclonal Antibodies Produced by Mammalian Cell Expression Systems
PurposeThis study aims to establish a benchmark glycan profile for commercial therapeutic monoclonal antibodies (mAbs) approved by the US Food and...
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Histone H3 proline 16 hydroxylation regulates mammalian gene expression
Histone post-translational modifications (PTMs) are important for regulating various DNA-templated processes. Here, we report the existence of a...
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Recombinant Protein Production in Suspension Mammalian Cells Using the BacMam Baculovirus Expression System
Mammalian cell lines are one of the best options when it comes to the production of complex proteins requiring specific glycosylation patterns....
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Expression and Purification of Mammalian NMDA Receptor Protein for Functional Characterization
N-methyl-D-aspartate (NMDA) receptors are critical for brain function and serve as drug targets for the treatment of neurological and psychiatric...
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CRISPR-Cas systems target endogenous genes to impact bacterial physiology and alter mammalian immune responses
CRISPR-Cas systems are an immune defense mechanism that is widespread in archaea and bacteria against invasive phages or foreign genetic elements. In...
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Transposable elements in mammalian chromatin organization
Transposable elements (TEs) are mobile DNA elements that comprise almost 50% of mammalian genomic sequence. TEs are capable of making additional...
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Mammalian Cell Culture: An Overview
The mammalian body typically consists of trillions of cells. These cells are the basic structural and functional units of a mammalian body. Together,...
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Epimorphic regeneration in the mammalian tympanic membrane
Adult mammals are generally believed to have limited ability to regenerate complex tissues and instead, repair wounds by forming scars. In humans and...
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Organization and expression of the mammalian mitochondrial genome
The mitochondrial genome encodes core subunits of the respiratory chain that drives oxidative phosphorylation and is, therefore, essential for energy...
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Expression of mammalian cell entry genes in clinical isolates of M. tuberculosis and the cell entry potential and immunological reactivity of the Rv0590A protein
Mammalian cell entry ( mce ) operons play a vital role in cell invasion and survival of M. tuberculosis . Of the mce genes, the function of Rv0590A is...
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Production of Secreted Antibody in Baculovirus Expression Vector System
Monoclonal antibodies have widespread applications in disease treatment and antigen detection. They are traditionally produced using mammalian cell...
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Microbial Contamination of Mammalian Cell Culture
Contamination is commonly used as a term for the presence of undesired microorganisms, unwanted mammalian cells, and various biochemical or chemicals...
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Extracellular release in the quality control of the mammalian mitochondria
Mammalian cells release a wealth of materials to their surroundings. Emerging data suggest these materials can even be mitochondria with perturbed...
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Mammalian Cells, Tissues and Organ Culture: Applications
In today’s world, mammalian cell cultures are used to understand various physiological and pathophysiological cell signaling events related to normal...
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Effects of marine biotoxins on drug-metabolizing cytochrome P450 enzymes and their regulation in mammalian cells
Marine biotoxins are a heterogenous group of natural toxins, which are able to trigger different types of toxicological responses in animals and...
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Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing
Methods for the targeted integration of genes in mammalian genomes suffer from low programmability, low efficiencies or low specificities. Here we...
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The Serotonin System in Mammalian Oogenesis
Transmitters, particularly serotonin, not only have the classical function of transmitting nerve impulses, but also take part in a whole series of...
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Large-Scale Culture of Mammalian Cells for Various Industrial Purposes
There is the widespread use of large-scale mammalian cell culture in biotechnological and pharmaceutical industries for various purposes. Select...
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Isolation and Primary Culture of Various Mammalian Cells
Isolation and primary culture of four major mammalian cells comprise the major contents of this chapter. The cells described here are endothelial...
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Isolation and Purification of Various Mammalian Cells: Single Cell Isolation
A typical adult mammalian body consists of 30–40 trillion cells. Mammalian cells differ in their shape, size, morphology, and other structural and...