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N-Glycan Insertion for Probing Protein–Protein Interactions and Epitope Map**
Introducing an N-linked glycosylation motif into recombinant proteins at specific sites is a useful tool in probing protein–protein interactions and...
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Engineering Selective TIMPs Using a Counter-Selective Screening Strategy
The yeast surface display platform provides a powerful approach for screening protein diversity libraries to identify binders with an enhanced...
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Design and Execution of In Vitro Polymerase Assays for Measles Virus and Related Mononegaviruses
Morbilliviruses such as measles virus (MeV) are responsible for major morbidity and mortality worldwide, despite the availability of an effective...
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Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase
Influenza A (FLUAV) and influenza B (FLUBV) viruses are human and/or animal pathogens widely studied due to their importance to public health and...
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Cronobacter sakazakii Infection in Early Postnatal Rats Impaired Contextual-Associated Learning: a Putative Role of C5a-Mediated NF-κβ and ASK1 Pathways
This study was designed to test whether the Cronobacter sakazakii infection-impaired contextual learning and memory are mediated by the activation of...
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Dissemination of the high-risk cloneST147 carbapenem-resistant klebsiella pneumoniae from a local tertiary care hospital in the Republic of Korea
BackgroundThe emergence of carbapenem-resistant Enterobacterales (CRE) infections is rapidly increasing and represents a serious public threat. In...
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Techniques for Genetic Manipulation
In the last few decades, molecular techniques and genetic modification have been used in genotype and phenotype studies of S. suis. Genomic...
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Introduce a novel post-biotic against Pseudomonas aeruginosa biofilm formation using Escherchia coli Nissle1917 outer membrane vesicles
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that can cause acute infections as well as chronic ones in humans. The expression of algD ...
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Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye
Infection by positive-strand RNA viruses induces extensive remodeling of the host endomembrane system in favor of viral replication and movement. The...
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Characterization of Tobacco Microbiome by Metagenomics Approach
Chronic consumption of tobacco in all forms, either smoked/smokeless forms, causes major health hazards to humans that include cancer,...
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Protocols for Isolation of Plasmid DNA
Plasmid DNA isolation is indivisible step in the development of diagnostic assays based on recombinant proteins and other molecular biology...
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Producing Biologics with Defined N-Glycosylation in Plants
The proper glycosylation of glycoproteins is important for their structure and function. This is an especially important consideration when choosing...
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Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules
Reconstitution of intracellular transport in cell-free in vitro assays enables the understanding and dissection of the molecular mechanisms that...
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Isolation, characterization and genomic analysis of the novel Arthrobacter sp. phage SWEP2
A new virulent phage, SWEP2, infecting the Arthrobacter sp. 5B strain, was isolated from black soil samples in northeastern China. SWEP2 has a latent...
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Observing Bacterial Persistence at Single-Cell Resolution
Within a bacterial population, there can be a subpopulation of cells with an antibiotic-tolerant persister phenotype characterized by long lag phase....
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Chromatographic isolation of potentially novel antibiotic compounds produced by Yimella sp. RIT 621
ObjectiveAntibiotic resistant infections have become a global health crisis causing 1.2 million deaths worldwide in 2019 [
1 ]. In a previous study,...
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Identification and characterization of two Bacillus anthracis bacteriophages
Anthrax is an acute infectious zoonotic disease caused by Bacillus anthracis , a bacterium that is considered a potential biological warfare agent. Bacillus...
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A Potent Drug l-Asparaginase from Marine Origins: A Comprehensive Review
l-Asparaginase (E.C.3.5.1.1) is a crucial enzyme with immense applications in the biomedical and food sectors. Due to its ability to convert the...
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Ice nucleation active bacteria metabolites as antibiofilm agent to control Aeromonas hydrophila and Streptococcus agalactiae infections in Aquaculture
ObjectivesThe aim of this study was to quantify and identify metabolites of Ice Nucleation Active (INA) bacteria as an anti-biofilm agent against...
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Optical Control of Mononegavirus Gene Expression and Replication
Mononegaviruses are promising tools as oncolytic and transgene vectors for gene therapy and regenerative medicine. However, when mononegaviruses are...