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Digital Quantification of Human Viral RNA and DNA Using a Self-Digitization Chip
Digital nucleic acid quantitation methods show excellent sensitivity and specificity for pathogen detection. Droplet digital PCR (ddPCR) is the most...
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Droplet-Based Microfluidic Chip Design, Fabrication, and Use for Ultrahigh-Throughput DNA Analysis and Quantification
DNA is widely used as a biomarker of contamination, infection, or disease, which has stimulated the development of a wide palette of detection and...
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DNA Fragment Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP)
Chromatin immunoprecipitation (ChIP) is a versatile method to investigate the interaction between specific proteins and DNA regions in vivo. The...
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Chromatin Immunoprecipitation Assay in Mouse Retinal Tissue
Chromatin immunoprecipitation (ChIP) is one of the most widely used methods for investigating interactions between proteins and DNA sequences. ChIP...
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Chromatin Immunoprecipitation Sequencing (ChIP-Seq) Assay in Food Allergy Research
Chromatin immunoprecipitation (ChIP) analysis followed by genome-wide sequencing represents the most efficient method for studying the complex...
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The Chromatin Immunoprecipitation (ChIP) Protocol for African Turquoise Killifish
DNA-binding proteins and epigenetic modifications coordinate to regulate the spatiotemporal pattern of gene expression during development, and thus,...
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Chromatin Immunoprecipitation from Formaldehyde Cross-Linked Olfactory Sensory Neurons
Chromatin immunoprecipitation (ChIP) allows a researcher to determine the genomic occupancy of nuclear proteins, providing insight into the roles of...
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Clonal hematopoiesis is associated with protection from Alzheimer’s disease
Clonal hematopoiesis of indeterminate potential (CHIP) is a premalignant expansion of mutated hematopoietic stem cells. As CHIP-associated mutations...
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Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples
Cell-free DNA has many applications in clinical medicine, in particular in cancer diagnosis and cancer treatment monitoring. Microfluidic-based...
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Cancer Sample Analysis Utilizing Single-Nucleotide Polymorphism Array and Array Comparative Genomic Hybridization
Chromosomal microarray, including single-nucleotide polymorphism (SNP) array and array comparative genomic hybridization (aCGH), enables the...
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Targeted Modification of Epigenetic Marks Using CRISPR/dCas9-SunTag-Based Modular Epigenetic Toolkit
The epigenome, consisting of chemical modifications to DNA and histone proteins, can alter gene expression. Clustered regularly interspaced short...
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Nucleosome reorganisation in breast cancer tissues
BackgroundNucleosome repositioning in cancer is believed to cause many changes in genome organisation and gene expression. Understanding these...
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CHIP promotes CAD ubiquitination and degradation to suppress the proliferation and colony formation of glioblastoma cells
PurposeCancer cells are characterized as the uncontrolled proliferation, which demands high levels of nucleotides that are building blocks for DNA...
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Multiomic profiling of transcription factor binding and function in human brain
Transcription factors (TFs) orchestrate gene expression programs crucial for brain function, but we lack detailed information about TF binding in...
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A hybrid RNA FISH immunofluorescence protocol on Drosophila polytene chromosomes
ObjectivesInvestigating protein-DNA interactions is imperative to understanding fundamental concepts such as cell growth, differentiation, and cell...
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Quantitative imaging of doxorubicin diffusion and cellular uptake in biomimetic gels with human liver tumor cells
Novel tumor-on-a-chip approaches are increasingly used to investigate tumor progression and potential treatment options. To improve the effect of any...
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Genome-wide distribution of histone trimethylation reveals a global impact of bisphenol A on telomeric binding proteins and histone acetyltransferase factors: a pilot study with human and in vitro data
ObjectiveTo assess the genetic and epigenetic effects promoted by Bisphenol A (BPA) exposure in adolescent males from the Spanish INMA-Granada birth...
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Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY
Studies of DNA–protein interactions have revealed regulatory mechanisms of DNA replication, repair, remodeling, and transcription. Perturbation of...
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Identification of DNA methylation biomarkers for evaluating cardiovascular disease risk from epigenome profiles altered by low-dose ionizing radiation
BackgroundEnvironmental exposure, medical diagnostic and therapeutic applications, and industrial utilization of radionuclides have prompted a...
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Meta-analysis of integrated ChIP-seq and transcriptome data revealed genomic regions affected by estrogen receptor alpha in breast cancer
BackgroundThe largest group of patients with breast cancer are estrogen receptor-positive (ER + ) type. The estrogen receptor acts as a transcription...
