Search
Search Results
-
Enhanced Pharmacokinetic Bioanalysis of Antibody–drug Conjugates using Hybrid Immunoaffinity Capture and Microflow LC-MS/MS
The increasing complexity and diversity of antibody–drug conjugates (ADCs) have led to a need for comprehensive and informative bioanalytical methods...
-
Antibody Immobilization
In the ELISA format(s), the capture antibody is usually affixed to a solid phase, commonly referred to as the immunosorbent. How to tether the...
-
Antigen–Antibody Reactions
The interactions between antigens and antibodies are known as antigen–antibody reactions. The reactions are highly specific, and an antigen reacts...
-
Production of Neutralizing Antibody
Techniques employing monoclonal antibodies (mAbs) are widely used in the initial development phase of biologics. The usefulness of mAbs in basic RA...
-
Overcoming Soluble Target Interference in Measurement of Total Bispecific Therapeutic Antibody Concentrations
The measurement of therapeutic drug concentrations is used to assess drug exposure and the relationship between therapeutic pharmacokinetics (PK) and...
-
PI3Kγ in B cells promotes antibody responses and generation of antibody-secreting cells
The differentiation of naive and memory B cells into antibody-secreting cells (ASCs) is a key feature of adaptive immunity. The requirement for...
-
A minimal physiologically based pharmacokinetic model to study the combined effect of antibody size, charge, and binding affinity to FcRn/antigen on antibody pharmacokinetics
Protein therapeutics have revolutionized the treatment of a wide range of diseases. While they have distinct physicochemical characteristics that...
-
ADAMTS13 Antibody and Inhibitor Assays
A finding of an ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type 1 motif, member 13) activity level of <10% of normal is...
-
Molecular Dynamics Methods for Antibody Design
Complex and coordinated dynamics are closely connected with protein functions, including the binding of antibodies to antigens. Knowledge of such...
-
Evolution of Antidrug Antibody Assays During the Development of Anti-Tissue Factor Pathway Inhibitor Monoclonal Antibody Marstacimab
Tissue factor pathway inhibitor (TFPI) is an endogenous inhibitor of the extrinsic coagulation pathway. In patients with hemophilia A or B,...
-
Current Trends in Validating Antibody Specificities for ELISpot by Western Blotting
The enzyme-linked immunospot (ELISpot) assay is a highly useful and sensitive method to detect total immunoglobulin and antigen-specific...
-
Antibody-displaying extracellular vesicles for targeted cancer therapy
Extracellular vesicles (EVs) function as natural delivery vectors and mediators of biological signals across tissues. Here, by leveraging these...
-
Identification, sorting and profiling of functional killer cells via the capture of fluorescent target-cell lysate
Assays for assessing cell-mediated cytotoxicity are largely target-cell-centric and cannot identify and isolate subpopulations of cytotoxic effector...
-
Pharmacokinetics and Pharmacodynamics of TAK-164 Antibody Drug Conjugate Coadministered with Unconjugated Antibody
The development of new antibody–drug conjugates (ADCs) has led to the approval of 7 ADCs by the FDA in 4 years. Given the impact of intratumoral...
-
Successful Development of Nonclinical Anti-Drug Antibody Assays to Support Zinpentraxin Alfa Reproductive Toxicology Studies
Immunogenicity assessment is an essential part of biotherapeutic drug development. While the immune response in animals is not always representative...
-
A candidate antibody drug for prevention of malaria
Over 75% of malaria-attributable deaths occur in children under the age of 5 years. However, the first malaria vaccine recommended by the World...
-
Analytical Method for Experimental Validation of Computer-Designed Antibody
In the computational design of antibodies, the interaction analysis between target antigen and antibody is an essential process to obtain feedback...
-
Addressing Domain Specificity in the Development of a Cell-Based Binding Assay for the Detection of Neutralizing Antibodies Against a CD47xPD-L1 Bispecific Antibody
PF-07257876 is a bispecific antibody being developed for the treatment of certain advanced or metastatic solid tumors. To support clinical...
-
Deep Learning in Therapeutic Antibody Development
Deep learning applied to antibody developmentAntibody development is in its adolescence. Low data volumes and biological platform differences make...
-
Computational optimization of antibody humanness and stability by systematic energy-based ranking
Conventional methods for humanizing animal-derived antibodies involve grafting their complementarity-determining regions onto homologous human...
