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Computational Analysis of lncRNA from cDNA Sequences
Based on recent findings, long noncoding (lnc) RNAs represent a potential class of functional molecules within the cell. In this chapter we describe...
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Long-term memory T cells as preventive anticancer immunity elicited by TuA-derived heteroclitic peptides
The host’s immune system may be primed against antigens during the lifetime (e.g. microorganisms antigens—MoAs), and swiftly recalled upon growth of...
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Activity-Dependent Labeling of Olfactory Sensory Neurons Using RNA Fluorescence In Situ Hybridization Followed by Phospho-S6 Immunofluorescence
This microscope-based method allows demonstrating that an odorant receptor responded to an odorant in vivo. In sections of olfactory epithelium from...
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Strain Construction for Intracellular Metabolic Pathway Localization in Y. lipolytica
Pathway localization by fluorophore or epitope tagging can be accomplished through a multi-staged DNA construct and confirmation process, to generate...
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Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing
Bacterial transposons propagate through either non-replicative (cut-and-paste) or replicative (copy-and-paste) pathways, depending on how the mobile...
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Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast
ObjectiveThe objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA...
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Genetic Engineering of Therapeutic Phages Using Type III CRISPR-Cas Systems
The functional characterization of “hypothetical” phage genes is a major bottleneck in basic and applied phage research. To compound this issue, the...
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Implementing CRISPR-Cas12a for Efficient Genome Editing in Yarrowia lipolytica
The oleaginous yeast Yarrowia lipolytica has emerged as an industrially relevant chassis to produce various valuable chemicals. Metabolic engineering...
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Homeoprotein SIX1 compromises antitumor immunity through TGF-β-mediated regulation of collagens
The tumor microenvironment (TME), including infiltrated immune cells, is known to play an important role in tumor growth; however, the mechanisms...
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Enhanced antitumour response of gold nanostar-mediated photothermal therapy in combination with immunotherapy in a mouse model of colon carcinoma
ObjectivesThis study investigated the potential of combining PTT with dendritic cell (DC)-based immunotherapy and anti-PD-L1 immune checkpoint...
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Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity
Anti-tumour inflammatory cytokines are highly toxic when administered systemically. Here, in multiple syngeneic mouse models, we show that the...
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Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway
BackgroundTumor-associated macrophages (TAMs), which form a large part of the tumor microenvironment, are normally regulated by metabolic...
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The artificial amino acid change in the sialic acid-binding domain of the hemagglutinin neuraminidase of newcastle disease virus increases its specificity to HCT 116 colorectal cancer cells and tumor suppression effect
BackgroundOncolytic viruses are being studied and developed as novel cancer treatments. Using directed evolution technology, structural modification...
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ARF6 promotes hepatocellular carcinoma proliferation through activating STAT3 signaling
BackgroundHepatocellular Carcinoma (HCC) possesses the high mortality in cancers worldwide. Nevertheless, the concrete mechanism underlying HCC...
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Wnt/β-catenin signalling activates IMPDH2-mediated purine metabolism to facilitate oxaliplatin resistance by inhibiting caspase-dependent apoptosis in colorectal cancer
BackgroundOxaliplatin resistance usually leads to therapeutic failure and poor prognosis in colorectal cancer (CRC), while the underlying mechanisms...
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Oleuropein-driven reprogramming of the myeloid cell compartment to sensitise tumours to PD-1/PD-L1 blockade strategies
BackgroundPrevious studies have shown that functional systemic immunity is required for the efficacy of PD-1/PD-L1 blockade immunotherapies in...
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To fight or not to fight: activation of the mPFC during decision to engage in aggressive behavior after ethanol consumption in a novel murine model
RationaleAlcohol consumption is a common antecedent of aggressive behavior. The effects of alcohol on the decision to engage in aggression in...
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Lnc-LRRTM4 promotes proliferation, metastasis and EMT of colorectal cancer through activating LRRTM4 transcription
Numerous mechanisms have shown that long noncoding RNAs (lncRNAs) promote the development of colorectal cancer (CRC), but the role of lnc-LRRTM4 in...
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Knockout of a key gene of the nicotine biosynthetic pathway severely affects tobacco growth under field, but not greenhouse conditions
ObjectiveThere is great interest in develo** tobacco plants containing minimal amounts of the addictive compound nicotine. Quinolate...
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Nano-optogenetic CAR-T Cell Immunotherapy
Chimeric antigen receptor (CAR)-T cell immunotherapy emerges as an effective cancer treatment. However, significant safety concerns remain, such as...