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Neutrophil–macrophage communication via extracellular vesicle transfer promotes itaconate accumulation and ameliorates cytokine storm syndrome
Cytokine storm syndrome (CSS) is a life-threatening systemic inflammatory syndrome involving innate immune hyperactivity triggered by various...
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Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment
Intravital microscopy is widely used for in vivo studies of the mechanisms of carcinogenesis and response to antitumor therapy. For visualization of...
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Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility
The ability to study the microcirculation in real time is key to elucidating how the immune system and the associated microvasculature interact and...
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A Novel Mouse Model for Cerebral Venous Sinus Thrombosis
Cerebral venous sinus thrombosis (CVST) is an uncommon cause of stroke resulting in parenchymal injuries associated with heterogeneous clinical...
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Novel application of live imaging to determine the functional cell biology of endothelial-to-mesenchymal transition (EndMT) within a liver-on-a-chip platform
ObjectiveImaging endothelial cell behaviour under physiological conditions, particularly those associated with chronic fibrotic pathologies, is an...
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Evaluation of Vascular Permeability in Inflamed Vessels of the Cremaster Muscle in Live Mice
Inflammation in vascular structures due to external factors such as injury or infection inevitably leads to blood leakage. Therefore, measuring blood...
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Staining MIF in Cells for Confocal Microscopy
Confocal microscopy is a powerful technique for immunofluorescence imaging of cells and tissues. The technique allows for detailed analysis of...
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New approaches for the assessment of platelet activation status in thrombus under flow condition using confocal microscopy
The goal of the study was the assessment of heterogeneous platelet activation status in thrombus. In a ferric(III) chloride (FeCl 3 ) thrombosis...
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Real-time imaging of mitochondrial redox reveals increased mitochondrial oxidative stress associated with amyloid β aggregates in vivo in a mouse model of Alzheimer’s disease
BackgroundReactive oxidative stress is a critical player in the amyloid beta (Aβ) toxicity that contributes to neurodegeneration in Alzheimer’s...
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Intravital Imaging of Vaccinia Virus-Infected Mice
Intravital multiphoton microscopy (MPM) allows the direct visualization of viral infections in real time as they occur in living animals. Here we...
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Dynamic Immunotherapy Study in Brain Tumor-Bearing Mice
We describe here a method to follow mouse glioma progression and its immune profile over time by intravital multiphoton microscopy. After craniotomy...
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Multimodal evaluation of an interphotoreceptor retinoid-binding protein-induced mouse model of experimental autoimmune uveitis
We aimed to characterize the vascular phenotypes of an experimental autoimmune retinal uveitis (EAU) model induced by interphotoreceptor...
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Advances in Tumor Microenvironment Immune Profiling
During the past decade, an abundance of scientific advancements have been made in the field of immunology. With these advancements, greater...
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CAR T-cell behavior and function revealed by real-time imaging
Adoptive transfer of T-cells expressing chimeric antigen receptors (CAR) has shown remarkable clinical efficacy against advanced B-cell malignancies....
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Visualizing and Subty** Tumor Ecosystem
A revolutionary way of classifying malignancies based on molecular markers and classification models is referred to as “molecular subty** of...
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Changes in intrathoracic pressure, not arterial pulsations, exert the greatest effect on tracer influx in the spinal cord
BackgroundCerebrospinal fluid (CSF) circulation in the brain has garnered considerable attention in recent times. In contrast, there have been fewer...
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Interleukin-6 drives endothelial glycocalyx damage in COVID-19 and bacterial sepsis
Damage of the endothelial glycocalyx (eGC) plays a central role in the development of vascular hyperpermeability and organ damage during systemic...
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MRI-Based and Histologically Verified 3D Modeling of Spatial Distribution of Intra-Arterially Transplanted Cells in Rat Brain
Visualization of transplanted stem cells in the brain is an important issue in the study of the mechanisms of their therapeutic action. MRI allowing...
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Optical Approaches to Understanding Enteric Circuits Along the Radial Axis
GutGutMicrobesMealsGutIntestinal epitheliumEnteric nervous system (ENS)Intrinsic primary afferent neurons (IPANs)Intrinsic primary afferent neurons...
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Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer
Ovarian cancer (OVCA) is frequently detected at late stages of disease, often with dissemination throughout the peritoneal cavity surface, abdomen,...