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IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling
Interleukin-17 (IL-17 or IL-17A) production is a hallmark of TH17 cells, a new unique lineage of CD4+ T lymphocytes contributing to the pathogenesis of multiple autoimmune and inflammatory diseases. IL-17 recepto...
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Article
Immunogenicity of induced pluripotent stem cells
Induced pluripotent stem (iPS) cells, which are produced by reprogramming fully differentiated adult cells back to an embryonic-like state by expression of specific genes, have important therapeutic potential....
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Open AccessGenetic approach to track neural cell fate decisions using human embryonic stem cells
With their capability to undergo unlimited self-renewal and to differentiate into all cell types in the body, human embryonic stem cells (hESCs) hold great promise in human cell therapy. However, there are lim...
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Open AccessHomologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template
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Open AccessLive cell imaging of genomic loci using dCas9-SunTag system and a bright fluorescent protein
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Open AccessGene activation in human cells using CRISPR/Cpf1-p300 and CRISPR/Cpf1-SunTag systems
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Open AccessCorrection to: Gene activation in human cells using CRISPR/Cpf1-p300 and CRISPR/Cpf1-SunTag systems
In the original publication the Supplementary Material and Fig. 2 are incorrect. The correct version is provided in this correction article. The text HBG2 appearing in the article should be read as HBG1.
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Article
Open AccessNOS1 inhibits the interferon response of cancer cells by S-nitrosylation of HDAC2
The dysfunction of type I interferon (IFN) signaling is an important mechanism of immune escape and metastasis in tumors. Increased NOS1 expression has been detected in melanoma, which correlated with dysfunct...
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Open AccessCell-cell contact-induced gene editing/activation in mammalian cells using a synNotch-CRISPR/Cas9 system
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Open AccessThe role of S-nitrosylation of PFKM in regulation of glycolysis in ovarian cancer cells
One of the malignant transformation hallmarks is metabolism reprogramming, which plays a critical role in the biosynthetic needs of unchecked proliferation, abrogating cell death programs, and immunologic esca...
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Article
Open AccessSOX9 inactivation affects the proliferation and differentiation of human lung organoids
The regulation of the transcription factor sex-determining region Y-box transcription factor 9 (SOX9) in lung development has been described in mouse, but the same principles apply to human lung development is...
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Article
Open AccessSingle-cell RNA-seq reveals fibroblast heterogeneity and increased mesenchymal fibroblasts in human fibrotic skin diseases
Fibrotic skin disease represents a major global healthcare burden, characterized by fibroblast hyperproliferation and excessive accumulation of extracellular matrix. Fibroblasts are found to be heterogeneous i...
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Article
Open AccessTag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
Genome-wide identification of DNA double-strand breaks (DSBs) induced by CRISPR-associated protein (Cas) systems is vital for profiling the off-target events of Cas nucleases. However, current methods for off-...
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Open AccessOSMRβ mutants enhance basal keratinocyte differentiation via inactivation of the STAT5/KLF7 axis in PLCA patients
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Open AccessHost metabolism dysregulation and cell tropism identification in human airway and alveolar organoids upon SARS-CoV-2 infection
The coronavirus disease 2019 (COVID-19) pandemic is caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is spread primary via respiratory droplets and infects the l...
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Article
Open AccessEngineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity
The CRISPR-Cas12a (formerly Cpf1) system is a versatile gene-editing tool with properties distinct from the broadly used Cas9 system. Features such as recognition of T-rich protospacer-adjacent motif (PAM) and...
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Article
Open AccessSLC35E1 promotes keratinocyte proliferation in psoriasis by regulating zinc homeostasis
Keratinocyte hyperproliferation is a key pathogenic factor in psoriasis. However, the mechanisms that regulate keratinocyte hyperproliferation in this condition remain unclear. Here, we found that SLC35E1 was ...
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Article
Open AccessEngineered circular guide RNAs boost CRISPR/Cas12a- and CRISPR/Cas13d-based DNA and RNA editing
The CRISPR/Cas12a and CRISPR/Cas13d systems are widely used for fundamental research and hold great potential for future clinical applications. However, the short half-life of guide RNAs (gRNAs), particularly ...
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Open AccessShort cell-penetration peptide conjugated bioreducible polymer enhances gene editing of CRISPR system
CRISPR-based gene therapy offers precise targeting and specific editing of disease-related gene sequences, potentially yielding long-lasting treatment effects. However, efficient delivery remains a significant...