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Article
Porcine phosphotyrosine interaction domain containing 1 modulates 3T3-L1 preadipocyte proliferation and differentiation
The phosphotyrosine interaction domain containing 1 (PID1) gene was firstly isolated from obese subjects and involved in obesity-associated insulin resistance. In the present study, Duroc×Landrace×Yorkshire (DLY)...
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Article
Prokaryotic expression and characterization of a keratinolytic protease from Aspergillus niger
In this study, a keratinolytic protease gene (named as kerD) from Aspergillus niger was cloned. The full-length coding sequence of kerD consists of 1,251 bp and encodes a protein with 416 amino acid residues with...
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Article
Codon optimization of Aspergillus niger feruloyl esterase and its expression in Pichia pastoris
To enhance the expression level of recombinant feruloyl esterase in Pichia pastoris, the Aspergillus niger feruloyl esterase (AnFaeA) gene was optimized based on the codon usage bias of P. pastoris and synthesize...
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Article
Role of FIT2 in porcine intramuscular preadipocyte differentiation
Fat-inducing transcript 2 (FIT2) plays an important role in the formation of intramuscular lipid droplets in skeletal muscle. However, its role in porcine intramuscular preadipocyte differentiation remains unc...
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Article
Open AccessDietary pea fiber increases diversity of colonic methanogens of pigs with a shift from Methanobrevibacter to Methanomassiliicoccus-like genus and change in numbers of three hydrogenotrophs
Pea fiber (PF) is a potential fibrous supplement in swine production. The influence of dietary PF on microbial community in the colon of pigs remains largely unexplored. Methanogens in the hindgut of monogastr...
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Article
Arginine induces skeletal muscle fiber type conversion by upregulating Akirin2 and AMPK/PGC-1α in mice
We have previously reported that arginine promotes slow myosin heavy chain expression in cell culture via Akirin2 and the AMP-activated protein kinase (AMPK) signaling pathway. Our goal in the present study wa...