12 Result(s)
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Article
Protective effect of Bombyx mori gloverin on intestinal epithelial cells exposure to enterotoxigenic E. coli
Bombyx mori gloverin A2 (BMGlvA2) is an induced antimicrobial insect protein isolated from Bombyx mori. This study was conducted to explore the effect and potential mechanisms of BMGlvA2 on inflammatory responses...
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Article
Human β-Defensin 118 Attenuates Escherichia coli K88–Induced Inflammation and Intestinal Injury in Mice
Antibiotics are widely used to treat various inflammatory bowel diseases caused by enterotoxigenic Escherichia coli (ETEC). However, continuous use of antibiotics may lead to drug resistance. In this study, we in...
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Article
Changes of porcine gut microbiota in response to dietary chlorogenic acid supplementation
Chlorogenic acids (CGA), the most abundant natural polyphenol present in human diet and plants, have attracted considerable research interest because of their broad bioactivities including the antimicrobial ac...
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Arginine induces skeletal muscle fiber type conversion by upregulating Akirin2 and AMPK/PGC-1α in mice
We have previously reported that arginine promotes slow myosin heavy chain expression in cell culture via Akirin2 and the AMP-activated protein kinase (AMPK) signaling pathway. Our goal in the present study wa...
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Fungi in Gastrointestinal Tracts of Human and Mice: from Community to Functions
Fungi are often ignored in studies on gut microbes because of their low level of presence (making up only 0.1% of the total microorganisms) in the gastrointestinal tract (GIT) of monogastric animals. Recent st...
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Open AccessDietary pea fiber increases diversity of colonic methanogens of pigs with a shift from Methanobrevibacter to Methanomassiliicoccus-like genus and change in numbers of three hydrogenotrophs
Pea fiber (PF) is a potential fibrous supplement in swine production. The influence of dietary PF on microbial community in the colon of pigs remains largely unexplored. Methanogens in the hindgut of monogastr...
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Article
Role of FIT2 in porcine intramuscular preadipocyte differentiation
Fat-inducing transcript 2 (FIT2) plays an important role in the formation of intramuscular lipid droplets in skeletal muscle. However, its role in porcine intramuscular preadipocyte differentiation remains unc...
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Article
Codon optimization of Aspergillus niger feruloyl esterase and its expression in Pichia pastoris
To enhance the expression level of recombinant feruloyl esterase in Pichia pastoris, the Aspergillus niger feruloyl esterase (AnFaeA) gene was optimized based on the codon usage bias of P. pastoris and synthesize...
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Article
Unique sequence characteristics account for good DGGE separation of almost full-length 18S rDNAs
A major limiting factor for DGGE-based microbial community studies is that the fragments should not be much longer than 500 bp for successful analysis. However, relatively high-resolution was achieved based on...
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Article
Prokaryotic expression and characterization of a keratinolytic protease from Aspergillus niger
In this study, a keratinolytic protease gene (named as kerD) from Aspergillus niger was cloned. The full-length coding sequence of kerD consists of 1,251 bp and encodes a protein with 416 amino acid residues with...
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Article
Porcine phosphotyrosine interaction domain containing 1 modulates 3T3-L1 preadipocyte proliferation and differentiation
The phosphotyrosine interaction domain containing 1 (PID1) gene was firstly isolated from obese subjects and involved in obesity-associated insulin resistance. In the present study, Duroc×Landrace×Yorkshire (DLY)...
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Article
Expression of a keratinase (kerA) gene from Bacillus licheniformis in Escherichia coli and characterization of the recombinant enzymes
The gene kerA (1,047 bp) encoding the main keratinase from Bacillus licheniformis was cloned into two conventional vectors, pET30α and pET32α, and expressed in Escherichia coli. From SDS-PAGE analysis, the recomb...
