5 Result(s)
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Article
Open AccessIdentification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms
Fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid (TCA) cycle and in amino acid metabolism. Fumarase is also used for the industrial production of L
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Article
Open AccessHyperproduction of poly(4-hydroxybutyrate) from glucose by recombinant Escherichia coli
Poly(4-hydroxybutyrate) [poly(4HB)] is a strong thermoplastic biomaterial with remarkable mechanical properties, biocompatibility and biodegradability. However, it is generally synthesized when 4-hydroxybutyra...
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Article
Open AccessProduction of copolyesters of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by E. coli containing an optimized PHA synthase gene
Microbial polyhydroxyalkanoates (PHA) are biopolyesters consisting of diverse monomers. PHA synthase PhaC2Ps cloned from Pseudomonas stutzeri 1317 is able to polymerize short-chain-length (scl) 3-hydroxybutyrate ...
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Article
Open AccessGenetically sha** morphology of the filamentous fungus Aspergillus glaucus for production of antitumor polyketide aspergiolide A
For filamentous fungi, the basic growth unit of hyphae usually makes it sensitive to shear stress which is generated from mechanical force and dynamic fluid in bioreactor, and it severely decreases microbial p...
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Article
Open AccessA strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation
With the rapid development of synthetic biology, the demand for assembling multiple DNA (genes) fragments into a large circular DNA structure in one step has dramatically increased. However, for constructions ...
