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11 Result(s)

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  1. No Access

    Article

    The highly conserved negatively charged Glu141 and Asp145 of the G-protein-coupled receptor RXFP3 interact with the highly conserved positively charged arginine residues of relaxin-3

    Relaxin-3 is a newly identified insulin/relaxin superfamily peptide that plays a putative role in the regulation of food intake and stress response by activating its cognate G-protein-coupled receptor RXFP3. R...

    Wei-Jie Zhang, **n-Yi Wang, Yu-Qi Guo, **ao Luo, Xue-Juan Gao, **ao-**a Shao… in Amino Acids (2014)

  2. No Access

    Article

    Secretory overexpression and isotopic labeling of the chimeric relaxin family peptide R3/I5 in Pichia pastoris

    Relaxin family peptides are a group of peptide hormones with divergent biological functions. Mature relaxin family peptides are typically composed of two polypeptide chains with three disulfide linkages, rende...

    Yu-Qi Guo, Qing-** Wu, **ao-**a Shao, Ting Shen, Ya-Li Liu, Zeng-Guang Xu… in Amino Acids (2015)

  3. No Access

    Article

    Novel bioluminescent receptor-binding assays for peptide hormones: using ghrelin as a model

    Peptide hormones perform important biological functions by binding specific cell membrane receptors. For hormone–receptor interaction studies, receptor-binding assays are widely used. However, conventional rec...

    Yu Liu, **ao-**a Shao, Lei Zhang, Ge Song, Ya-Li Liu, Zeng-Guang Xu… in Amino Acids (2015)

  4. No Access

    Article

    Application of the novel bioluminescent ligand–receptor binding assay to relaxin-RXFP1 system for interaction studies

    Relaxin is a prototype of the relaxin family peptide hormones and plays important biological functions by binding and activating the G protein-coupled receptor RXFP1. To study their interactions, in the presen...

    Qing-** Wu, Lei Zhang, **ao-**a Shao, Jia-Hui Wang, Yu Gao, Zeng-Guang Xu… in Amino Acids (2016)

  5. No Access

    Article

    Identification of hydrophobic interactions between relaxin-3 and its receptor RXFP3: implication for a conformational change in the B-chain C-terminus during receptor binding

    Relaxin-3 is an insulin/relaxin superfamily neuropeptide implicated in the regulation of food intake and stress response via activation of the G protein-coupled receptor RXFP3. Their electrostatic interactions...

    Meng-Jun Hu, **ao-**a Shao, Jia-Hui Wang, Dian Wei, Ya-Li Liu, Zeng-Guang Xu… in Amino Acids (2016)

  6. No Access

    Article

    A novel BRET-based binding assay for interaction studies of relaxin family peptide receptor 3 with its ligands

    Relaxin family peptide receptor 3 (RXFP3) is an A-class G protein-coupled receptor that is implicated in the regulation of food intake and stress response upon activation by its cognate agonist relaxin-3. To s...

    Jia-Hui Wang, **ao-**a Shao, Meng-Jun Hu, Dian Wei, Ya-Li Liu, Zeng-Guang Xu… in Amino Acids (2017)

  7. No Access

    Article

    Rapid preparation of bioluminescent tracers for relaxin family peptides using sortase-catalysed ligation

    Relaxin family is a group of peptide hormones with a variety of biological functions by activating G protein-coupled receptors RXFP1–4. We recently developed bioluminescent tracers for their receptor-binding a...

    Jia-Hui Wang, **ao-**a Shao, Meng-Jun Hu, Dian Wei, Wei-Han Nie, Ya-Li Liu… in Amino Acids (2017)

  8. No Access

    Article

    Development of a novel ligand binding assay for relaxin family peptide receptor 3 and 4 using NanoLuc complementation

    Relaxin family peptides perform a variety of biological functions by binding and activating relaxin family peptide receptor 1–4 (RXFP1–4), four A-class G protein-coupled receptors. In the present work, we deve...

    Meng-Jun Hu, **ao-**a Shao, Hao-Zheng Li, Wei-Han Nie, Jia-Hui Wang… in Amino Acids (2018)

  9. No Access

    Article

    Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5

    The insulin superfamily is a group of homologous proteins that are further divided into the insulin family and relaxin family according to their distinct receptors. All insulin superfamily members contain thre...

    Jia-Hui Wang, **ao-**a Shao, Meng-Jun Hu, Ya-Li Liu, Zeng-Guang Xu… in Amino Acids (2019)

  10. No Access

    Article

    LEAP2 has antagonized the ghrelin receptor GHSR1a since its emergence in ancient fish

    Recent studies have demonstrated that liver-expressed antimicrobial peptide 2 (LEAP2) antagonizes the ghrelin receptor GHSR1a in mammals. However, its antagonistic function in lower vertebrates has not yet bee...

    Hao-Zheng Li, Li-Li Shou, **ao-**a Shao, Ning Li, Ya-Li Liu, Zeng-Guang Xu… in Amino Acids (2021)

  11. No Access

    Article

    Nanomolar range of FAM237B can activate receptor GPR83

    Our recent study confirmed that the mature neuropeptide FAM237A, also known as neurosecretory protein GL (NPGL), is an efficient agonist for GPR83. The paralog FAM237B was previously reported as a weak agonist...

    Hao-Zheng Li, Ya-Fen Wang, Wen-Feng Hu, Ya-Li Liu, Zeng-Guang Xu… in Amino Acids (2023)