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  1. Article

    Open Access

    Metabolic engineering of Synechococcus elongatus PCC 7942 for improvement of 1,3-propanediol and glycerol production based on in silico simulation of metabolic flux distribution

    Production directly from carbon dioxide by engineered cyanobacteria is one of the promising technologies for sustainable future. Previously, we have successfully achieved 1,3-propanediol (1,3-PDO) production u...

    Yasutaka Hirokawa, Shingo Matsuo, Hiroyuki Hamada… in Microbial Cell Factories (2017)

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    Article

    Structural and physiological studies on the storage β-polyglucan of haptophyte Pleurochrysis haptonemofera

    The storage β-polyglucan and catabolic enzyme activities of the haptophyte Pleurochrysis haptonemofera were characterized. The storage β-polyglucan was prepared by the dimethylsulfoxide-extraction method. 13C- an...

    Yasutaka Hirokawa, Shoko Fujiwara, Motoya Suzuki, Tomoka Akiyama, Minako Sakamoto… in Planta (2008)

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    Article

    Gene Expression Profiling of Coccolith-Bearing Cells and Naked Cells in Haptophyte Pleurochrysis haptonemofera with a cDNA Macroarray System

    Pleurochrysis haptonemofera is a unicellular marine coccolithophorid that has calcified scales, coccoliths, on the cell surface. Some coccolithophorids including P. haptonemofera have a c...

    Shoko Fujiwara, Yasutaka Hirokawa, Yukiko Takatsuka, Kunihiro Suda… in Marine Biotechnology (2007)

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    Article

    Protoplast Formation of the Coccolithophorid Pleurochrysis haptonemofera in Hypoosmotic K+ Solution: Shedding of the Coccosphere and Regrowth of the Protoplast in Normal Medium

    Both coccolith-bearing cells (C-cells) and naked cells (N-cells) of the coccolithophorid Pleurochrysis haptonemofera can grow in salinities of more than 7‰ (about 20% of a “normal” sea water salinity [35‰]), with...

    Takatoshi Takayanagi, Yasutaka Hirokawa, Maki Yamamoto… in Marine Biotechnology (2007)

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    Article

    Three Types of Acidic Polysaccharides Associated with Coccolith of Pleurochrysis haptonemofera: Comparison with Pleurochrysis carterae and Analysis Using Fluorescein-Isothiocyanate-Labeled Lectins

    In the coccolithophorid microalgae acidic polysaccharides are considered to be involved in the formation of the calcified scale, coccolith. Characteristics of the acidic polysaccharides extracted from the cell...

    Yasutaka Hirokawa, Shoko Fujiwara, Mikio Tsuzuki in Marine Biotechnology (2005)

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    Article

    Discrimination of the Cell Surface of the Coccolithophorid Pleurochrysis haptonemofera from Light Scattering and Fluorescence After Fluorescein-Isothiocyanate-Labeled Lectin Staining Measured by Flow Cytometry

    We investigated the extent of calcification on the cell surface of the coccolithophorid Pleurochrysis haptonemofera using flow cytometry. Side scattering (SSC) by coccolith-bearing cells was higher than that by ...

    Jun-ichiro Takahashi, Shoko Fujiwara, Masami Kikyo… in Marine Biotechnology (2002)