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Article
Exopolysaccharide: a novel important factor in the microbial dissolution of tricalcium phosphate
Four strains (Enterobacter sp. EnHy-401, Arthrobacter sp.ArHy-505, Azotobacter sp.AzHy-510 and Enterobacter sp.EnHy-402) which have the ability to solubilize tricalcium phosphate (TCP) were used to study the mech...
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Article
Development and application of a loop-mediated isothermal amplification method on rapid detection of Pseudomonas aeruginosa strains
We developed and evaluated the specificity and sensitivity of a simple loop-mediated isothermal amplification (LAMP) method for rapid detection of P. aeruginosa strains. The optimal reaction condition was found t...
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Article
Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells
The expression of the human insulin-like growth factor (hIGF-I) gene driven by the Fhx/P25 promoter in the silk glands of transgenic silkworms (Bombyx mori) and in transformed silkworm cells, was achieved using B...
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Article
Open AccessRapid and simple detection of methicillin-resistance staphylococcus aureus by orfXloop-mediated isothermal amplification assay
Methicillin-resistant Staphylococcus aureus (MRSA) has become one of the most prevalent pathogens responsible for nosocomial infections throughout the world. As clinical MRSA diagnosis is concerned, current diagn...
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Article
Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production
To enhance the thermal and alkaline pH stability of the lysine decarboxylase from Escherichia coli (CadA) by engineering the decameric interface and explore its potential for industrial applications.
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Article
Open AccessDevelopment of a baculovirus vector carrying a small hairpin RNA for suppression of sf-caspase-1 expression and improvement of recombinant protein production
The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and in...
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Article
Open AccessGREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli
Late-stage fermentation broth contains high concentrations of target chemicals. Additionally, it contains various cellular metabolites which have leaked from lysed cells, which would exert multifactorial stres...
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Article
Circular RNA circ_0032821 contributes to oxaliplatin (OXA) resistance of gastric cancer cells by regulating SOX9 via miR-515-5p
Chemoresistance is one of the major obstacles for gastric cancer (GC) treatment. Exosome-mediated transfer of circular RNAs (circRNAs) is associated with the drug-resistance in GC. Circ_0032821 has been report...
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Article
Changes in soil ammonia oxidizers and potential nitrification after clear-cutting of boreal forests in China
The Korean pine and broad-leaved mixed forests are the most typical and complete ecosystem among the global boreal forests, with extremely important ecological functions. However, few studies on the changes of...
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Article
Open AccessSpatial distribution pattern across multiple microbial groups along an environmental stress gradient in tobacco soil
It has become commonplace to explore the spatial distribution patterns of microbial communities in natural ecosystems. However, few have looked at the responses of community diversity, structure, and assembly ...
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Article
Purification and enzymatic properties of a new thermostable endoglucanase from Aspergillus oryzae HML366
Aspergillus oryzae HML366 is a newly screened cellulase-producing strain. The endoglucanase HML ED1 from A. oryzae HML366 was quickly purified by a two-step method that combines ammonium sulfate precipitation and...
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Article
Whole genome analysis of Pseudomonas mandelii SW-3 and the insights into low-temperature adaptation
Pseudomonas mandelii SW-3, isolated from the Napahai plateau wetland, can survive in cold environments. The mechanisms underlying the survival of bacteria in low temperatures and high altitudes are not yet fully ...