-
Article
Open AccessThe contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons
Clostridium thermocellum is a thermophilic anaerobic bacterium that degrades cellulose by using a highly effective cellulosome, a macromolecular complex consisting of multiple cellulose degrading enzymes organize...
-
Article
Open AccessA novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum
Clostridium cellulolyticum and other cellulolytic Clostridium strains are natural producers of lignocellulosic biofuels and chemicals via the consolidated bioprocessing (CBP) route, and systems...
-
Article
Open AccessEfficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotei...
-
Article
Open AccessDetermination of the native features of the exoglucanase Cel48S from Clostridium thermocellum
Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C. thermocellum, Cel48S plays key roles...
-
Article
Open AccessFirmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique + 1 transcriptional slippage
Bacterial insertion sequences (ISs) are ubiquitous mobile genetic elements that play important roles in genome plasticity, cell adaptability, and function evolution. ISs of various families and subgroups conta...
-
Article
Open AccessConstruction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for ...
-
Article
Open AccessBioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils
Lignocellulose is a valuable carbon source for the production of biofuels and biochemicals, thus having the potential to substitute fossil resources. Consolidated bio-saccharification (CBS) is a whole-cell-bas...