59 Result(s)
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Article
Open AccessEnhancing the expression of the unspecific peroxygenase in Komagataella phaffii through a combination strategy
The unspecific peroxygenase (UPO) from Cyclocybe aegerita (AaeUPO) can selectively oxidize C–H bonds using hydrogen peroxide as an oxygen donor without cofactors, which has drawn significant industrial attention....
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Article
Metabolic engineering of Corynebacterium glutamicum for the efficient production of β-Alanine from glucose
β-Alanine is the only naturally occurring β-type amino acid, with various applications in the pharmaceutical, food, and chemical industries. Given the growing market demand, the study of β-alanine production i...
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Genome-wide analysis of in vivo-evolved Candida auris reveals multidrug-resistance mechanisms
Candida auris, an emerging and multidrug-resistant fungal pathogen, has led to numerous outbreaks in China. While the resistance mechanisms against azole and amphotericin B have been studied, the development of d...
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Open AccessIdentification of a novel thermostable transaminase and its application in L-phosphinothricin biosynthesis
Transaminase (TA) is a crucial biocatalyst for enantioselective production of the herbicide L-phosphinothricin (L-PPT). The use of enzymatic cascades has been shown to effectively overcome the unfavorable ther...
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Article
Open AccessSpecific gastrointestinal microbiota profiles in Chinese Tan sheep are associated with lauric acid content in muscle
The biological mechanisms underlying meat quality remain unclear. Currently, many studies report that the gastrointestinal microbiota is essential for animal growth and performance. However, it is uncertain wh...
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Novel β1,4 N-acetylglucosaminyltransferase in de novo enzymatic synthesis of hyaluronic acid oligosaccharides
The efficiency of de novo synthesis of hyaluronic acid (HA) using Pasteurella multocida hyaluronate synthase (PmHAS) is limited by its low catalytic activity during the initial reaction steps when monosaccharides...
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Crypt-like patterned electrospun nanofibrous membrane and probiotics promote intestinal epithelium models close to tissues
In vitro intestinal epithelium models have drawn great attention to investigating intestinal biology in recent years. However, the difficulty to maintain the normal physiological status of primary intestinal e...
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Research progress of l-aspartate-α-decarboxylase and its isoenzyme in the β-alanine synthesis
Driven by the massive demand in recent years, the production of β-alanine has significantly progressed in chemical and biological ways. Although the chemical method is relatively mature compared to biological ...
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Open AccessThe numbers of fungi: contributions from traditional taxonomic studies and challenges of metabarcoding
The global diversity of fungi has been estimated using several different approaches. There is somewhere between 2–11 million estimated species, but the number of formally described taxa is around 150,000, a ti...
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Expression of l-phosphinothricin synthesis enzymes in Pichia pastoris for synthesis of l-phosphinothricin
With the ban of highly toxic herbicides, such as paraquat and glyphosate, phosphinothricin (PPT) is becoming the most popular broad-spectrum and highly effective herbicide. The current PPT products in the mark...
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Article
Engineering laboratory/factory-specific phage-resistant strains of Escherichia coli by mutagenesis and screening
The Escherichia coli (E. coli) has been widely employed in biotechnology industry and academia. However, the bioproduct manufacturing driven by E. coli is prone to the phage contamination. Good laboratory/factory...
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Open AccessInvestigation of hub gene associated with the infection of Staphylococcus aureus via weighted gene co-expression network analysis
Staphylococcus aureus is a gram-positive bacterium that causes serious infection. With the increasing resistance of bacteria to current antibiotics, it is necessary to learn more about the molecular mechanism an...
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Biological synthesis of nicotinamide mononucleotide
Nicotinamide mononucleotide (NMN) or Nicotinamide-1-ium-1-β-D-ribofuranoside 5′-phosphate is a nucleotide that can be converted into nicotinamide adenine dinucleotide (NAD) in human cells. NMN has recently att...
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Isolation and Identification of Pseudomonas sp. Strain DY-1 from Agricultural Soil and Its Degradation Effect on Prometryne
Prometryne is a widely used herbicide in China to control annual grasses and broadleaf weeds. However, the stability of prometryne makes it difficult to be degraded, which poses a threat to human health. This ...
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Article
Identification of a novel promoter for driving antibiotic-resistant genes to reduce the metabolic burden during protein expression and effectively select multiple integrations in Pichia Pastoris
Routine approaches for the efficient expression of heterogenous proteins in Pichia pastoris include using the strong methanol-regulated alcohol oxidase (AOX1) promoter and multiple inserts of expression cassettes...
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Article
Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress
Butanol inhibits bacterial activity by destroying the cell membrane of Clostridium acetobutylicum strains and altering functionality. Butanol toxicity also results in destruction of the phosphoenolpyruvate-carboh...
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Open AccessMesenchymal stem cells desensitize castration-resistant prostate cancer to docetaxel chemotherapy via inducing TGF-β1-mediated cell autophagy
Mesenchymal stem cells (MSCs) have been proved to drive castration resistant prostate cancer (CRPC). In this study, we aim to investigate the contribution of MSCs to the development of docetaxel resistance in ...
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Article
High throughput solid-phase screening of bacteria with cyclic amino alcohol deamination activity for enantioselective synthesis of chiral cyclic β-amino alcohols
To screening of bacteria with cyclic amino alcohol deamination activity for enantioselective synthesis of chiral cyclic β-amino alcohols.
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Article
Fluorescence-based high-throughput screening system for R-ω-transaminase engineering and its substrate scope extension
ω-Transaminase (ω-TA) is an attractive alternative to metal catalysts for the stereoselective amination of prochiral ketones. The narrow substrate scope of an R-ω-transaminase from Mycobacterium vanbaalenii (MvTA...
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Article
Engineering a Pichia pastoris nitrilase whole cell catalyst through the increased nitrilase gene copy number and co-expressing of ER oxidoreductin 1
1-Cyanocyclohexaneacetic acid (1-CHAA) is a critical intermediate for the synthesis of the antiepileptic agent gabapentin. Previously, our group has established a novel manufacturing route for 1-CHAA through b...
