55 Result(s)
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Article
Open AccessOptimizing enzyme properties to enhance dihydroxyacetone production via methylglyoxal biosensor development
Dihydroxyacetone (DHA) stands as a crucial chemical material extensively utilized in the cosmetics industry. DHA production through the dephosphorylation of dihydroxyacetone phosphate, an intermediate product ...
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Open Access3D printed zirconia used as dental materials: a critical review
In view of its high mechanical performance, outstanding aesthetic qualities, and biological stability, zirconia has been widely used in the fields of dentistry. Due to its potential to produce suitable advance...
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Article
Open AccessResearch progress of biomimetic materials in oral medicine
Biomimetic materials are able to mimic the structure and functional properties of native tissues especially natural oral tissues. They have attracted growing attention for their potential to achieve configurab...
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Analysis of endophytic bacterial flora of mulberry cultivars susceptible and resistant to bacterial wilt using metagenomic sequencing and culture-dependent approach
Endophytes have a wide range of potential in maintaining plant health and sustainable agricultural environmental conditions. In this study, we analysed the diversity of endophytic bacteria in four mulberry cul...
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Open AccessA CRISPR-based chromosomal-separation technique for Escherichia coli
Natural life systems can be significantly modified at the genomic scale by human intervention, demonstrating the great innovation capacity of genome engineering. Large epi-chromosomal DNA structures were estab...
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Open AccessHeterologous expression of pediocin/papA in Bacillus subtilis
Listeria monocytogenes is a food-borne pathogen. Pediocin is a group IIα bacteriocin with anti-listeria activity that is naturally produced by Pediococcus acidilactic and Lactobacillus plantarum. The pedA/papA ge...
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Open AccessImproving astaxanthin production in Escherichia coli by co-utilizing CrtZ enzymes with different substrate preference
The bifunctional enzyme β-carotene hydroxylase (CrtZ) catalyzes the hydroxylation of carotenoid β-ionone rings at the 3, 3’ position regardless of the presence of keto group at 4, 4’ position, which is an impo...
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Article
Open AccessFine-tuning the expression of pathway gene in yeast using a regulatory library formed by fusing a synthetic minimal promoter with different Kozak variants
Tailoring gene expression to balance metabolic fluxes is critical for the overproduction of metabolites in yeast hosts, and its implementation requires coordinated regulation at both transcriptional and transl...
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Open AccessMetabolic engineering of Escherichia coli for de novo production of 3-phenylpropanol via retrobiosynthesis approach
3-Phenylpropanol with a pleasant odor is widely used in foods, beverages and cosmetics as a fragrance ingredient. It also acts as the precursor and reactant in pharmaceutical and chemical industries. Currently...
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Article
Expression and kinetic analysis of carboxylesterase LmCesA1 from Locusta migratoria
To investigate the biochemical characterization of the carboxylesterase LmCesA1 from Locusta migratoria.
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Open AccessComparative genomics and transcriptomics analyses provide insights into the high yield and regulatory mechanism of Norvancomycin biosynthesis in Amycolatopsis orientalis NCPC 2-48
Norvancomycin has been widely used in clinic to treat against MRSA (Methicillin-resistant Staphylococcus aureus) and MRSE (Methicillin-resistant Staphylococcus epidermidis) infections in China. Amycolatopsis orie...
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Article
Curcusone C induces apoptosis in endometrial cancer cells via mitochondria-dependent apoptotic and ERK pathway
Jatropha curcashas been used in traditional medicine in Africa to treat cancer for thousands of years. This study aimed to examine the anti-endometrial cancer effect of Curcusone C, a naturally occurring rhamnofo...
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Open AccessManipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency
Deactivated Cas9 (dCas9) led to significant improvement of CRISPR/Cas9-based techniques because it can be fused with a variety of functional groups to form diverse molecular devices, which can manipulate or mo...
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Open AccessEngineering the Calvin–Benson–Bassham cycle and hydrogen utilization pathway of Ralstonia eutropha for improved autotrophic growth and polyhydroxybutyrate production
CO2 is fixed by all living organisms with an autotrophic metabolism, among which the Calvin–Benson–Bassham (CBB) cycle is the most important and widespread carbon fixation pathway. Thus, studying and engineering ...
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Open AccessIsolation, heterologous expression, and purification of a novel antifungal protein from Bacillus subtilis strain Z-14
Wheat sheath blight, a soil borne fungal disease caused by Rhizoctonia cerealis, is considered as one of the most serious threats to wheat worldwide. Bacillus subtilis Z-14 was isolated from soil sampled from a w...
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Improving the thermostability of a GH97 dextran glucosidase by rational design
This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using ...
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Open AccessA programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3)
Escherichia coli BL21 is arguably the most popular host for industrial production of proteins, and industrial fermentations are often plagued by phage infections. The CRISPR/Cas system is guided by a gRNA to clea...
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Open AccessIdentification of new arylamine N-acetyltransferases and enhancing 2-acetamidophenol production in Pseudomonas chlororaphis HT66
2-Acetamidophenol (AAP) is an aromatic compound with the potential for antifungal, anti-inflammatory, antitumor, anti-platelet, and anti-arthritic activities. Due to the biosynthesis of AAP is not yet fully un...
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Improve uridine production by modifying related metabolic pathways in Bacillus subtilis
The metabolic pathway related to uridine production was modified in Bacillus subtilis in order to increase the production of uridine.
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Article
Improving RNA content of salt-tolerant Zygosaccharomyces rouxii by atmospheric and room temperature plasma (ARTP) mutagenesis and its application in soy sauce brewing
Derived from RNA, 5ʹ-ribonucleotides, especially Inosine-5ʹ-monophosphate (IMP) and guanosine-5ʹ-monophosphate (GMP), can enhance the umami taste of soy sauce. In this study, the RNA content of three different...
