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Article
The first genetic map of a synthesized allohexaploid Brassica with A, B and C genomes based on simple sequence repeat markers
We present the first genetic map of an allohexaploid Brassica species, based on segregating microsatellite markers in a doubled haploid map** population ge...
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Article
Molecular cloning and characterization of gloverin from the diamondback moth, Plutella xylostella L. and its interaction with bacterial membrane
Gloverin restricted to Lepidoptera is known to be a glycine-rich and heat stable antimicrobial protein. The current research reports a 650 bp full-length cDNA encoding gloverin from Plutella xylostella (PxGlo) b...
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Article
QTL detected for grain-filling rate in maize using a RIL population
The grain-filling rate plays an important role in determining grain yield. To elucidate the genetic basis of the grain-filling rate, a set of 203 recombinant inbred lines was evaluated at two locations over 2 ...
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Article
Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species
Twelve new LMW-GS genes were characterized from bread wheat (Triticum aestivum L.) cultivar Zhongyou 9507 and five Aegilops species by AS-PCR. These genes belong to the LMW-m type and can be classified into two s...
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Chapter and Conference Paper
Protoplast Technology and Citrus Improvement
Citrus is one most important fruit crop in China and worldwide. Protoplast fusion has been an effective and successful technique for citrus improvement by circumventing reproductive barriers such a...
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Article
Isolation of cytoplasts from Satsuma mandarin (Citrus unshiu Marc.) and production of alloplasmic hybrid calluses via cytoplast–protoplast fusion
Cytoplasm of Satsuma mandarin (Citrus unshiu Marc.) is known to influence seedlessness. Transfer of cytoplasm to a seedy cultivar could possibly lead to the production of seedless citrus fruits. In the present pa...
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Article
Variation in quality traits in common wheat as related to Chinese fresh white noodle quality
The variation in grain compositional traits related to Chinese fresh white noodle (CFWN) quality was examined in 25 leading Chinese common wheat cultivars and advanced lines. Large variations were observed in ...
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Article
GISH, AFLP and PCR-RFLP analysis of an intergeneric somatic hybrid combining Goutou sour orange and Poncirus trifoliata
Intergeneric somatic hybrids combining Goutou sour orange (Citrus aurantium L.) with trifoliate orange [Poncirus trifoliata (L.) Raf] were produced by electrofusion and their genetic inheritance analyzed by ampli...
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Article
Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness
CMS (cytoplasmic male sterility) can be controlled by the mitochondrion genome in higher plants, including Satsuma mandarin. Somatic fusion experiments in citrus combining embryogenic callus protoplasts of one...
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Article
Cell size as a morphological marker to calculate the mitotic index and ploidy level of citrus callus
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Article
Cell size as a morphological marker to calculate the mitotic index and ploidy level of citrus callus
We have developed a new method for calculating the mitotic index (MI) and ploidy level of citrus callus. The first step was to symbolize the diameters of new daughter cells and metaphase cells with R D and R M, ...
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Article
Wide somatic hybrids of Citrus with its related genera and their potential in genetic improvement
Citrus wild relatives are an untapped germplasm reservoir, which possesses many elite resistance traits. Genetic introgression into Citrus by conventional methods has been greatly hindered by polyembryony, pollen...
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Article
Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C. paradisi) for seedless triploid breeding
Protoplasts from cell suspension cultures of ‘Bonnaza’ navel orange (Citrus sinensis (L.) Osbeck) were electrically fused with mesophyll protoplasts isolated from seedless ‘Red Blush’ grapefruit (Citrusparadisi)....
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Article
Metal-induced expressing of mammal Metallothionein-1 gene in cyanobacteria to promote cadmium-binding preferences
The metal(zinc)-inducible smtA gene promoter (smt O-P) from cyanobacteria was applied for the expression of mouse MT-1 cDNA in the filamentous cyanobacterium Anabaena sp. PCC 7120 to enhance its metal-binding ca...
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Article
Intertribal hexaploid somatic hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium
Chinese wampee [Clausena lansium (Lour.) Skeels], a sexually incompatible relative of citrus, is commercially cultivated in South China. In this study, embryogenic protoplasts of ‘Bonanza’ navel orange [Citrus s...
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Article
Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion
Protoplasts isolated from `Page' tangelo (Minneola tangelo × clementine) cell suspension cultures were electrically fused with mesophyll protoplasts of orange jessamine [Murraya paniculata (L.) Jack]. Shoots wer...
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Article
Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets
Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige ...
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Article
Induction of triploid Citrus plants from endosperm calli in vitro
Triploid hybrid Citrus plants were regenerated by somatic embryogenesis in vitro from endosperm derived calli. A sequence of media formulations was used to induce and support proliferation of primary callus from ...